Fas Expression and Induction of Apoptosis
| UPN . | Fas (%) . | ΔN (%) . | Δ200 (%) . | Δ1000 (%) . | CFU-AML (−) . | CFU-AML (+) . |
|---|---|---|---|---|---|---|
| 2-18 | 11.8 | 4 | 4.1 | 15.2 | 0 | 0 |
| 1-5 | 13.4 | 8 | −2.9 | 3.9 | 63 | 58.5 |
| 1-9 | 15.4 | ND | −1.5 | −1.3 | 0 | 0 |
| 4-14 | 17.1 | ND | 0.6 | 1.7 | 29.5 | 5 |
| 1-8 | 17.5 | ND | −3.5 | −2.1 | 0 | 0 |
| 4-13 | 22.2 | 7 | 7.0 | 18.9 | 113 | 132.5 |
| 2-21 | 24.7 | 8 | 10.4 | 19.8 | ND | ND |
| 2-14 | 26.6 | −5 | ND | 5.9 | ND | ND |
| 4-6 | 34.1 | −1 | 0 | 5.6 | 23.5 | 21 |
| 2-16 | 38.6 | 0 | 0.1 | 1.6 | ND | ND |
| 4-11 | 41.6 | ND | 1.5 | 2.8 | ND | ND |
| 3-6 | 41.6 | ND | ND | 1.1 | ND | ND |
| 5-8 | 45.0 | −1 | 0.6 | 1.3 | 0 | 0 |
| 2-9 | 45.1 | 1 | 7.5 | 24.9 | 20 | 6.5 |
| 4-4 | 54.4 | 37 | 13.6 | 19.5 | 7.5 | |
| 2-12 | 55.9 | 9 | ND | 14.7 | 247 | 5.5 |
| 5-10 | 61.2 | ND | 0.3 | 9.1 | 0 | 0 |
| 4-9 | 65.1 | 22 | ND | 14.6 | ND | ND |
| 2-26 | 72.1 | 7 | −0.6 | 9.0 | 81 | 4.5 |
| 5-3 | 72.8 | 8 | ND | 10.4 | 21 | 13 |
| 2-22 | 73.1 | 0 | 7.5 | 7.3 | ND | ND |
| 3-5 | 77.1 | ND | 0.6 | 2.2 | ND | ND |
| 2-23 | 83.4 | 1 | 0.8 | 2.0 | 0 | 0 |
| 2-7 | 85.9 | 1 | 1.5 | 5.4 | 7 | 3 |
| UPN . | Fas (%) . | ΔN (%) . | Δ200 (%) . | Δ1000 (%) . | CFU-AML (−) . | CFU-AML (+) . |
|---|---|---|---|---|---|---|
| 2-18 | 11.8 | 4 | 4.1 | 15.2 | 0 | 0 |
| 1-5 | 13.4 | 8 | −2.9 | 3.9 | 63 | 58.5 |
| 1-9 | 15.4 | ND | −1.5 | −1.3 | 0 | 0 |
| 4-14 | 17.1 | ND | 0.6 | 1.7 | 29.5 | 5 |
| 1-8 | 17.5 | ND | −3.5 | −2.1 | 0 | 0 |
| 4-13 | 22.2 | 7 | 7.0 | 18.9 | 113 | 132.5 |
| 2-21 | 24.7 | 8 | 10.4 | 19.8 | ND | ND |
| 2-14 | 26.6 | −5 | ND | 5.9 | ND | ND |
| 4-6 | 34.1 | −1 | 0 | 5.6 | 23.5 | 21 |
| 2-16 | 38.6 | 0 | 0.1 | 1.6 | ND | ND |
| 4-11 | 41.6 | ND | 1.5 | 2.8 | ND | ND |
| 3-6 | 41.6 | ND | ND | 1.1 | ND | ND |
| 5-8 | 45.0 | −1 | 0.6 | 1.3 | 0 | 0 |
| 2-9 | 45.1 | 1 | 7.5 | 24.9 | 20 | 6.5 |
| 4-4 | 54.4 | 37 | 13.6 | 19.5 | 7.5 | |
| 2-12 | 55.9 | 9 | ND | 14.7 | 247 | 5.5 |
| 5-10 | 61.2 | ND | 0.3 | 9.1 | 0 | 0 |
| 4-9 | 65.1 | 22 | ND | 14.6 | ND | ND |
| 2-26 | 72.1 | 7 | −0.6 | 9.0 | 81 | 4.5 |
| 5-3 | 72.8 | 8 | ND | 10.4 | 21 | 13 |
| 2-22 | 73.1 | 0 | 7.5 | 7.3 | ND | ND |
| 3-5 | 77.1 | ND | 0.6 | 2.2 | ND | ND |
| 2-23 | 83.4 | 1 | 0.8 | 2.0 | 0 | 0 |
| 2-7 | 85.9 | 1 | 1.5 | 5.4 | 7 | 3 |
Δ, difference between the number of cells (%) cultured with anti-Fas MoAb and those cultured with control MoAb; ΔN, counted by morphological method, Δ200 and Δ1000; determined by colorimetric DNA fragmentation assay by flow cytometric analysis after the culture with anti-Fas MoAb at the concentration of 200 and 1,000 ng/mL, respectively.
Abbreviation: ND, not done.