Checkerboard Assay of Tonsillar B Cells in Response to rTNF
| Distance Migrated ( μm in 2 h) by Leading Front of Cell | |||
| rTNF (ng/mL) Below the Filter | |||
| 0 | 0.1 | 1 | |
| rTNF (ng/mL) above the filter: | |||
| 0 | 36.9 ± 4.6 | 54.1 ± 3.8 (38.7 ± 4.6) | 76.5 ± 11.2 (41.6 ± 6.9) |
| 0.1 | 38.8 ± 4.2 (43.4 ± 3.9) | 48.1 ± 3.5 | 55.9 ± 3.6 (49.3 ± 4.4) |
| 1 | 39.4 ± 1.1 (50.5 ± 6.1) | 38.0 ± 6.1 (52.3 ± 6.2) | 53.6 ± 7.0 |
| Distance Migrated ( μm in 2 h) by Leading Front of Cell | |||
| rTNF (ng/mL) Below the Filter | |||
| 0 | 0.1 | 1 | |
| rTNF (ng/mL) above the filter: | |||
| 0 | 36.9 ± 4.6 | 54.1 ± 3.8 (38.7 ± 4.6) | 76.5 ± 11.2 (41.6 ± 6.9) |
| 0.1 | 38.8 ± 4.2 (43.4 ± 3.9) | 48.1 ± 3.5 | 55.9 ± 3.6 (49.3 ± 4.4) |
| 1 | 39.4 ± 1.1 (50.5 ± 6.1) | 38.0 ± 6.1 (52.3 ± 6.2) | 53.6 ± 7.0 |
Results are expressed as mean ± SE, n = 3. Figures in parentheses show the calculated distance migrated if cells would have not responded to the gradient of rTNF, but only to absolute concentration.