bEnd-3 were grown to late postconfluence as described in the Materials and Methods. Monolayers were washed and refed fresh DMEM containing 1% BCS (serum-starved) or DMEM containing 10% BCS (serum-growing) and incubated for 24 hours before use. Medium was then removed and monolayers were replenished with serum-starved or serum-growing medium as indicated and activated with LPS (100 ng/mL) for 24 hours before harvesting RNA for Northern analysis. Representative results of CP-10 mRNA expression are shown.

Abbreviation: ND, not done.