Expression of HRX in Normal Human Tissues
| Tissue . | Reactivity With Anti-HRX Antibodies . |
|---|---|
| CNS | |
| Brain | Nuclei of neurons and microglia positive, endothelial cells weakly positive |
| Cerebellum | Strong staining of nuclei in granular and molecular layers |
| Cerebral cortex | Nuclei of neurons and microglia positive |
| Spinal cord | Nuclei staining of neuronal and glial cells |
| Gastrointestinal tract | |
| Colon | Nuclei of smooth muscle positive |
| Liver | Nuclei strongly positive with polyclonal antibody αHRX2. MoAb HRX 107 results in very weakly positive nuclei and some cross-reactivity with the cell membrane |
| Hematopoietic/lymphoid system | |
| Fetal liver | Nuclei positive with polyclonal, negative with monoclonal |
| Spleen | Nuclei of red and white pulp positive |
| Thymus | Nuclei positive particularly in endothelium and epithelium |
| Tonsil | Nuclei positive particularly in endothelium and epithelium |
| Urinary system | |
| Kidney | Nuclei in glomeruli and tubules positive |
| Circulatory system | |
| Heart | Nuclei positive |
| Endocrine system | |
| Thyroid gland | Nuclei positive |
| Respiratory system | |
| Lung | Nuclei positive, particularly macrophage nuclei |
| Reproductive system | |
| Testis | All nuclei strongly positive |
| Tissue . | Reactivity With Anti-HRX Antibodies . |
|---|---|
| CNS | |
| Brain | Nuclei of neurons and microglia positive, endothelial cells weakly positive |
| Cerebellum | Strong staining of nuclei in granular and molecular layers |
| Cerebral cortex | Nuclei of neurons and microglia positive |
| Spinal cord | Nuclei staining of neuronal and glial cells |
| Gastrointestinal tract | |
| Colon | Nuclei of smooth muscle positive |
| Liver | Nuclei strongly positive with polyclonal antibody αHRX2. MoAb HRX 107 results in very weakly positive nuclei and some cross-reactivity with the cell membrane |
| Hematopoietic/lymphoid system | |
| Fetal liver | Nuclei positive with polyclonal, negative with monoclonal |
| Spleen | Nuclei of red and white pulp positive |
| Thymus | Nuclei positive particularly in endothelium and epithelium |
| Tonsil | Nuclei positive particularly in endothelium and epithelium |
| Urinary system | |
| Kidney | Nuclei in glomeruli and tubules positive |
| Circulatory system | |
| Heart | Nuclei positive |
| Endocrine system | |
| Thyroid gland | Nuclei positive |
| Respiratory system | |
| Lung | Nuclei positive, particularly macrophage nuclei |
| Reproductive system | |
| Testis | All nuclei strongly positive |
Immunocytochemical analysis of freshly cut samples was found to be the most sensitive test for detecting HRX and it was apparent that if the material was not freshly prepared little or no staining was observed. Cell lines or sections which had been stored frozen for more than 2 to 3 months often showed little or no labeling of HRX. The staining was also fixation dependent, since harsher fixation, eg, paraformaldehyde, abolished staining.