Lung Metastasis in Control and Plg−/− Mice
| . | Metastatic Foci in Lungs at Day 21* . | |
|---|---|---|
| . | (median; range) . | |
| . | Control . | Plg−/− . |
| Primary tumor intact | Total† 350; 13->400‡ (8) | 361; 243->400‡ (7) |
| Primary tumor resected | ||
| Day 7 | Total† 14; 6-22 (4) | 13; 2-61 (10) |
| Day 10 | Total† 120; 43-251 (9) | 103; 57-182 (10) |
| Largeρ 38; 14-93 (10) | 43; 16-76 (10) | |
| . | Metastatic Foci in Lungs at Day 21* . | |
|---|---|---|
| . | (median; range) . | |
| . | Control . | Plg−/− . |
| Primary tumor intact | Total† 350; 13->400‡ (8) | 361; 243->400‡ (7) |
| Primary tumor resected | ||
| Day 7 | Total† 14; 6-22 (4) | 13; 2-61 (10) |
| Day 10 | Total† 120; 43-251 (9) | 103; 57-182 (10) |
| Largeρ 38; 14-93 (10) | 43; 16-76 (10) | |
Numbers in parentheses indicate number of mice used for determination of the value.
Lungs were fixed in Bouin's fixative and mechanically separated into individual lobes and metastatic foci were counted on each lobe.
Number of metastatic foci determined by counting under a stereo microscope at 4× magnification.
The lungs of 3 control mice and of 3 Plg−/− mice presented with too many metastatic foci for precise quantitation. The number of metastatic foci in these lungs was estimated to be between 400 and 500 and was assigned the value 400 in the determination of the median value.
ρ Number of metastatic foci determined by counting by naked eye.