A total of 180 Lin⁻Sca-1⁺c-kit⁺ cells were seeded at a concentration of 1 cell per well in complete IMDM and supplemented with FL + IL-7 + IL-10 at optimal concentrations. After 4 and 10 days of incubation, proliferating clones were identified (>2 cells) and KL + IL-3 + G-CSF added to stimulate myeloid differentiation. After an additional 10 days of incubation, clones were examined morphologically for myeloid cell production following Giemsa staining. Data represent the mean percentage (SD) of the fraction of clones with myeloid potential from three separate experiments. In comparison, 6 (2)% of the colonies formed from Lin⁻Sca-1⁺c-kit⁺ cells cultured throughout the incubation period with FL + IL-7 + IL-10 contained myeloid cells.