The Effect of Delayed Addition of IL-10 to FL + IL-7–Stimulated Lin−Sca-1+c-kit+ Progenitor Cells
| IL-10 . | Clone Size . | Total Clones . | |||
|---|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . | . |
| None | 10 (1) | 12 (1) | 9 (2) | 0 (1) | 32 (2) |
| 0 h | 8 (2) | 12 (3) | 15 (3)* | 16 (4)* | 49 (4)* |
| 24 h | 7 (2) | 7 (3)* | 12 (2) | 6 (3)* | 31 (3) |
| IL-10 . | Clone Size . | Total Clones . | |||
|---|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . | . |
| None | 10 (1) | 12 (1) | 9 (2) | 0 (1) | 32 (2) |
| 0 h | 8 (2) | 12 (3) | 15 (3)* | 16 (4)* | 49 (4)* |
| 24 h | 7 (2) | 7 (3)* | 12 (2) | 6 (3)* | 31 (3) |
Lin−Sca-1+c-kit+ cells were plated at 1 cell per well in 20 μL complete IMDM supplemented with FL + IL-7 or FL + IL-7 + IL-10. IL-10 was added either at initiation of culture (0 h) or following 24 hours (24 h) of incubation. After 10 days incubation at 37°C and 5% CO2 in air, clones were identified in an inverted microscope and scored according to the criteria described in Table 1. Results represent the mean (SEM) of three independent experiments with 180 cells plated for each group in each experiment.
P < .05 when compared with cultures in the absence of IL-10.