Table 1.

IL-10 Enhances the Growth of LinSca1+c-kit+ Bone Marrow Progenitor Cells

Clone SizeTotal
1234
Medium 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-10 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-7 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-7 + IL-10 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
FL 8 (1) 3 (2) 0 (0) 0 (0) 11 (4) 
FL + IL-10 4 (2) 16 (2) 9 (2) 0 (0) 28 (3)* 
KL 5 (1) 6 (2) 6 (1) 0 (0) 17 (3) 
KL + IL-10 6 (1) 12 (2) 10 (3) 0 (0) 27 (5)* 
FL + IL-7 9 (1) 14 (1) 9 (2) 0 (0) 32 (2) 
FL + IL-7 + IL-10 7 (3) 17 (3) 15 (3) 9 (3) 46 (3)* 
KL + IL-7 5 (1) 8 (3) 7 (2) 0 (1) 20 (6) 
KL + IL-7 + IL-10 6 (3) 16 (3) 20 (8) 5 (3) 47 (10)* 
Clone SizeTotal
1234
Medium 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-10 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-7 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
IL-7 + IL-10 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 
FL 8 (1) 3 (2) 0 (0) 0 (0) 11 (4) 
FL + IL-10 4 (2) 16 (2) 9 (2) 0 (0) 28 (3)* 
KL 5 (1) 6 (2) 6 (1) 0 (0) 17 (3) 
KL + IL-10 6 (1) 12 (2) 10 (3) 0 (0) 27 (5)* 
FL + IL-7 9 (1) 14 (1) 9 (2) 0 (0) 32 (2) 
FL + IL-7 + IL-10 7 (3) 17 (3) 15 (3) 9 (3) 46 (3)* 
KL + IL-7 5 (1) 8 (3) 7 (2) 0 (1) 20 (6) 
KL + IL-7 + IL-10 6 (3) 16 (3) 20 (8) 5 (3) 47 (10)* 

LinSca1+c-kit+ cells were seeded at one cell per well in 20 μL complete IMDM with cytokines as indicated. After 10 days of incubation at 37°C and 5% CO2 in air, cultures were scored for clonal growth according to the following criteria: 1 = 10 to 50 cells, 2 = 50 cells − 10% of the well covered with cells, 3 = 10% to 50% covered, and 4 = 50% to 100% of the well covered with cells. Results are presented as the mean (SEM) from three independent experiments with 180 wells scored per group in each experiment.

*

P < .05 for total clone numbers when compared with cultures in the absence of IL-10.

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