IL-10 Enhances the Growth of Lin−Sca1+c-kit+ Bone Marrow Progenitor Cells
. | Clone Size . | Total . | |||
---|---|---|---|---|---|
. | 1 . | 2 . | 3 . | 4 . | . |
Medium | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-10 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-7 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-7 + IL-10 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
FL | 8 (1) | 3 (2) | 0 (0) | 0 (0) | 11 (4) |
FL + IL-10 | 4 (2) | 16 (2) | 9 (2) | 0 (0) | 28 (3)* |
KL | 5 (1) | 6 (2) | 6 (1) | 0 (0) | 17 (3) |
KL + IL-10 | 6 (1) | 12 (2) | 10 (3) | 0 (0) | 27 (5)* |
FL + IL-7 | 9 (1) | 14 (1) | 9 (2) | 0 (0) | 32 (2) |
FL + IL-7 + IL-10 | 7 (3) | 17 (3) | 15 (3) | 9 (3) | 46 (3)* |
KL + IL-7 | 5 (1) | 8 (3) | 7 (2) | 0 (1) | 20 (6) |
KL + IL-7 + IL-10 | 6 (3) | 16 (3) | 20 (8) | 5 (3) | 47 (10)* |
. | Clone Size . | Total . | |||
---|---|---|---|---|---|
. | 1 . | 2 . | 3 . | 4 . | . |
Medium | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-10 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-7 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
IL-7 + IL-10 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
FL | 8 (1) | 3 (2) | 0 (0) | 0 (0) | 11 (4) |
FL + IL-10 | 4 (2) | 16 (2) | 9 (2) | 0 (0) | 28 (3)* |
KL | 5 (1) | 6 (2) | 6 (1) | 0 (0) | 17 (3) |
KL + IL-10 | 6 (1) | 12 (2) | 10 (3) | 0 (0) | 27 (5)* |
FL + IL-7 | 9 (1) | 14 (1) | 9 (2) | 0 (0) | 32 (2) |
FL + IL-7 + IL-10 | 7 (3) | 17 (3) | 15 (3) | 9 (3) | 46 (3)* |
KL + IL-7 | 5 (1) | 8 (3) | 7 (2) | 0 (1) | 20 (6) |
KL + IL-7 + IL-10 | 6 (3) | 16 (3) | 20 (8) | 5 (3) | 47 (10)* |
Lin−Sca1+c-kit+ cells were seeded at one cell per well in 20 μL complete IMDM with cytokines as indicated. After 10 days of incubation at 37°C and 5% CO2 in air, cultures were scored for clonal growth according to the following criteria: 1 = 10 to 50 cells, 2 = 50 cells − 10% of the well covered with cells, 3 = 10% to 50% covered, and 4 = 50% to 100% of the well covered with cells. Results are presented as the mean (SEM) from three independent experiments with 180 wells scored per group in each experiment.
P < .05 for total clone numbers when compared with cultures in the absence of IL-10.