Effects of DRB on Secretion of Ferritin and Albumin by Hepatoma Cells
| Treatment . | Ferritin . | Albumin . | ||
|---|---|---|---|---|
| . | Secretion . | Inhibition by DRB (%) . | Secretion . | Inhibition by DRB (%) . |
| . | (μg/mg cell protein) . | . | (μg/mg cell protein) . | . |
| Controls | ||||
| −DRB | 0.8 ± 0.0 | 53 ± 1 | ||
| +DRB | 0.02 ± 0.004-150 | 98 | 0.4 ± 0.04-150 | 99 |
| IL-1 | ||||
| −DRB | 1.9 ± 0.14-151 | 48 ± 1 | ||
| +DRB | 0.3 ± 0.04-150 | 86 | 0.4 ± 0.0 | 99 |
| TNF | ||||
| −DRB | 1.5 ± 0.04-151 | 45 ± 24-151 | ||
| +DRB | 0.1 ± 0.04-150 | 98 | 0.4 ± 0.0 | 99 |
| Fe-NTA | ||||
| −DRB | 4.5 ± 0.64-151 | 49 ± 3 | ||
| +DRB | 0.4 ± 0.04-150 | 91 | 0.5 ± 0.0 | 98 |
| Treatment . | Ferritin . | Albumin . | ||
|---|---|---|---|---|
| . | Secretion . | Inhibition by DRB (%) . | Secretion . | Inhibition by DRB (%) . |
| . | (μg/mg cell protein) . | . | (μg/mg cell protein) . | . |
| Controls | ||||
| −DRB | 0.8 ± 0.0 | 53 ± 1 | ||
| +DRB | 0.02 ± 0.004-150 | 98 | 0.4 ± 0.04-150 | 99 |
| IL-1 | ||||
| −DRB | 1.9 ± 0.14-151 | 48 ± 1 | ||
| +DRB | 0.3 ± 0.04-150 | 86 | 0.4 ± 0.0 | 99 |
| TNF | ||||
| −DRB | 1.5 ± 0.04-151 | 45 ± 24-151 | ||
| +DRB | 0.1 ± 0.04-150 | 98 | 0.4 ± 0.0 | 99 |
| Fe-NTA | ||||
| −DRB | 4.5 ± 0.64-151 | 49 ± 3 | ||
| +DRB | 0.4 ± 0.04-150 | 91 | 0.5 ± 0.0 | 98 |
Cells were grown almost to confluence in serum-containing medium, then switched to protein-free medium for 24 hours before harvest of cells and medium. Treatments with cytokines, iron, and DRB were for the final 24 hours. Doses of IL-1, TNF, and Fe-NTA were 5.0 ng/mL, 2.5 ng/mL, and 10 μg/mL, respectively, and the dose of DRB was 4 ng/mL. Data are means ± SD, for groups of three flasks that were treated identically. It is noteworthy that the treatments with DRB caused little or no change in cell number (as judged by recovery of cell protein from each flask) and no enhanced leakage of LDH.
Difference from −DRB is significant (P < .001).
Difference from control is significant (P < .001).