Effects of TNF and IL-1 on Release of Ferritin and Albumin From Hepatoma Cells
| . | Dose (ng/mL) . | Ferritin Released . | Albumin Released . | LDH (% of control) . | Cell Ferritin (μg/mg cell protein) . | ||
|---|---|---|---|---|---|---|---|
| . | . | (μg/mg cell prot.) . | (% of control) . | (μg/mg cell protein) . | (% of control) . | . | . |
| Controls (12) | 0 | 1.8 ± 0.3 | 100 ± 10 | 22 ± 1 | 100 ± 3 | 100 ± 23 | 10.1 ± 2.3 (6) |
| IL-1 (12) | 5.0, 10.0 | 4.8 ± 1.1* | 280 ± 75* | 19 ± 1* | 88 ± 3* | 121 ± 31 | 9.2 ± 0.6 (4) |
| TNF (12) | 2.5, 5.0 | 5.7 ± 2.0* | 329 ± 111* | 21 ± 1 | 97 ± 4 | 125 ± 10 | 11.2 ± 0.3 (4) |
| IL-1 + TNF (16) | All combinations | 9.5 ± 1.9*† | 526 ± 160*† | 19 ± 1* | 89 ± 5* | 138 ± 12* | 10.6 ± 4.5 (4) |
| . | Dose (ng/mL) . | Ferritin Released . | Albumin Released . | LDH (% of control) . | Cell Ferritin (μg/mg cell protein) . | ||
|---|---|---|---|---|---|---|---|
| . | . | (μg/mg cell prot.) . | (% of control) . | (μg/mg cell protein) . | (% of control) . | . | . |
| Controls (12) | 0 | 1.8 ± 0.3 | 100 ± 10 | 22 ± 1 | 100 ± 3 | 100 ± 23 | 10.1 ± 2.3 (6) |
| IL-1 (12) | 5.0, 10.0 | 4.8 ± 1.1* | 280 ± 75* | 19 ± 1* | 88 ± 3* | 121 ± 31 | 9.2 ± 0.6 (4) |
| TNF (12) | 2.5, 5.0 | 5.7 ± 2.0* | 329 ± 111* | 21 ± 1 | 97 ± 4 | 125 ± 10 | 11.2 ± 0.3 (4) |
| IL-1 + TNF (16) | All combinations | 9.5 ± 1.9*† | 526 ± 160*† | 19 ± 1* | 89 ± 5* | 138 ± 12* | 10.6 ± 4.5 (4) |
Rat hepatoma cells (H4-II-E-C3) were grown in horse and bobby calf serum containing Dulbecco's modified Eagle's medium for 3 days, then adapted to protein free (serum free) hybridoma medium for several hours. Individual 10 mL flasks of cells were then given hybridoma medium with or without various doses of lymphokines, as indicated, and harvested 48 hours later. Both medium and cells were assayed for ferritin and albumin by rocket immunoelectrophoresis. LDH was assayed to assess potential damage to cells. Data for the two most optimal doses of each lymphokine administered (and all combinations thereof) did not show a significant difference and were thus combined. Cells were harvested and assayed for ferritin and total protein. Values are means ± SD per mg cell protein for the number of flasks in parentheses. Statistical analysis was by t-test.
P < .01 or <.001 for difference from controls.
P < .01 or <.001 for difference from IL-1 or TNF alone.