Synthesis of Ferritin by Rat Hepatoma Cells in Culture
| . | Cell Medium . | Hepatoma Cells . |
|---|---|---|
| Total protein | ||
| Amount (mg) | 2,390 | 51 |
| Total 3H (cpm × 10−6) | 3.49 | 21.2 |
| Specific activity (cpm/mg) | 1,460 | 419,000 |
| Total ferritin isolated | ||
| Amount (μg) | 546 | 199 |
| Total 3H (cpm) | 1,821 | 387 |
| Specific activity (cpm/mg) | 3,336 | 1,946 |
| . | Cell Medium . | Hepatoma Cells . |
|---|---|---|
| Total protein | ||
| Amount (mg) | 2,390 | 51 |
| Total 3H (cpm × 10−6) | 3.49 | 21.2 |
| Specific activity (cpm/mg) | 1,460 | 419,000 |
| Total ferritin isolated | ||
| Amount (μg) | 546 | 199 |
| Total 3H (cpm) | 1,821 | 387 |
| Specific activity (cpm/mg) | 3,336 | 1,946 |
Ten flasks of cells were grown a total of 7 days in serum containing medium. Tracer 3H-leucine was added to the last change in medium, and the medium and cells were harvested 72 hours later. Ferritin was isolated from medium and cells, and the total radioactivity incorporated into the ferritin and total protein was measured.