Comparison of Synergistic Effects Between FL and SCF With IL-6/sIL-6R
| Factor(s) . | No. of Colonies per 500 CD34+ Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| FL + IL-6 + sIL-6R | 5 ± 0 | 8 ± 3 | 5 ± 1 | 28 ± 3 | 46 ± 3 |
| SCF + IL-6 + sIL-6R | 9 ± 1 | 5 ± 4 | 6 ± 3 | 85 ± 5 | 105 ± 6 |
| SCF + FL + IL-6 + sIL-6R | 10 ± 2 | 5 ± 2 | 4 ± 3 | 82 ± 8 | 101 ± 12 |
| Factor(s) . | No. of Colonies per 500 CD34+ Cells . | ||||
|---|---|---|---|---|---|
| . | GM . | MK . | B . | Mix . | Total . |
| FL + IL-6 + sIL-6R | 5 ± 0 | 8 ± 3 | 5 ± 1 | 28 ± 3 | 46 ± 3 |
| SCF + IL-6 + sIL-6R | 9 ± 1 | 5 ± 4 | 6 ± 3 | 85 ± 5 | 105 ± 6 |
| SCF + FL + IL-6 + sIL-6R | 10 ± 2 | 5 ± 2 | 4 ± 3 | 82 ± 8 | 101 ± 12 |
500 CD34+ cells were incubated in the presence of designated factor combinations in serum-containing culture, and the colonies were scored at day 14 of culture. Concentrations of cytokines were 100 ng/mL for FL, 100 ng/mL for SCF, 100 ng/mL for IL-6, and 1,000 ng/mL for sIL-6R. The number of colonies indicates mean ± SD of triplicate cultures.