Effect of Endogenous IL-10 on Cytokine Production
| Culture Conditions . | Enhancement (%) of Cytokine Production . | ||
|---|---|---|---|
| . | TNF-α . | IFN-γ . | IL-12 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-10R | 38 ± 13† | 31 ± 9* | 59 ± 51 |
| Anti-CD3 + IL-2 (100 ng/mL) + anti-IL-10R | 53 ± 16† | 52 ± 13* | 108 ± 64 |
| Culture Conditions . | Enhancement (%) of Cytokine Production . | ||
|---|---|---|---|
| . | TNF-α . | IFN-γ . | IL-12 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-10R | 38 ± 13† | 31 ± 9* | 59 ± 51 |
| Anti-CD3 + IL-2 (100 ng/mL) + anti-IL-10R | 53 ± 16† | 52 ± 13* | 108 ± 64 |
In the presence of accessory cells T cells were stimulated by anti-CD3 + IL-15 or IL-2 and an inhibitory anti-IL-10R MoAb (1 μg/mL) or a corresponding isotype control antibody (1 μg/mL). Depicted is the enhancement of TNF-α and IFN-γ production upon inhibition of endogenous IL-10 binding to the IL-10R. Mean and SEM of six independent experiments are shown. % Enhancement was calculated as (1 − [cytokine production in the presence of an anti-IL-10R MoAb]/[cytokine production in the presence of an isotype control antibody]). Stimulation of T cells with IL-2 in the presence of the control antibody revealed 185 ± 41 pg/mL TNF-α, 5.0 ± 1.1 ng/mL IFN-γ and 2.3 ± 1.4 pg/mL IL-12, while stimulation with IL-15 in the presence of the control antibody revealed 181 ± 35 pg/mL TNF-α, 4.8 ± 1.23 ng/mL IFN-γ, and 1.4 ± 0.15 pg/mL IL-12. Significant enhancement of cytokine production by blocking of IL-10 binding to its receptor is indicated by (* P < .01, † P < .025).