The Role of Endogenous IL-12 in IL-2– and IL-15–Mediated Cytokine Production
| Culture Conditions . | % Inhibition of Cytokine Production . | ||
|---|---|---|---|
| . | IFN-γ . | TNF-α . | IL-10 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-12 | 60.3 ± 4.4* | 32.3 ± 4.9† | 44.7 ± 6.1* |
| Anti-CD3 + IL-2 (100 ng/mL) + anti-IL-12 | 74 ± 2.3* | 27 ± 4.4† | 36 ± 1.1* |
| Culture Conditions . | % Inhibition of Cytokine Production . | ||
|---|---|---|---|
| . | IFN-γ . | TNF-α . | IL-10 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-12 | 60.3 ± 4.4* | 32.3 ± 4.9† | 44.7 ± 6.1* |
| Anti-CD3 + IL-2 (100 ng/mL) + anti-IL-12 | 74 ± 2.3* | 27 ± 4.4† | 36 ± 1.1* |
T cells were stimulated in the presence of accessory cells by anti-CD3 + IL-2 or IL-15 and a neutralizing anti-IL-12 antibody or an isotype control antibody. % Inhibition of cytokine production in the presence of the neutralizing MoAb was calculated (1 − [cytokine production in the presence of anti-IL-12]/[cytokine production in the presence of the isotype control antibody]) × 100%. Depicted are mean and SEM of three independent experiments. Significant inhibition of cytokine production is indicated by * (P < .01) or † (P < .025).