The Role of Endogenous IL-2 in IL-15–Mediated Cytokine Production
| Culture Conditions . | % Inhibition of Cytokine Production . | ||
|---|---|---|---|
| . | IFN-γ . | TNF-α . | IL-10 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-2R | 1 ± 9 | 1 ± 7.5 | 1 ± 7.6 |
| Anti-CD3 + IL-2 (10 ng/mL) + anti-IL-2R | 64 ± 8* | 68 ± 3.2* | 50 ± 5.4* |
| Anti-CD3 + IL-2 (1 ng/mL) + anti-IL-2R | 85 ± 9.5* | 90 ± 3.4* | 80 ± 4.8* |
| Culture Conditions . | % Inhibition of Cytokine Production . | ||
|---|---|---|---|
| . | IFN-γ . | TNF-α . | IL-10 . |
| Anti-CD3 + IL-15 (100 ng/mL) + anti-IL-2R | 1 ± 9 | 1 ± 7.5 | 1 ± 7.6 |
| Anti-CD3 + IL-2 (10 ng/mL) + anti-IL-2R | 64 ± 8* | 68 ± 3.2* | 50 ± 5.4* |
| Anti-CD3 + IL-2 (1 ng/mL) + anti-IL-2R | 85 ± 9.5* | 90 ± 3.4* | 80 ± 4.8* |
T cells were stimulated by anti-CD3 + IL-15 or IL-2 in the presence of an inhibitory MoAb against the high affinity-IL-2R or an isotype control antibody. % Inhibition of cytokine production in the presence of the inhibitory MoAb was calculated by (1 − [cytokine production in the presence of anti-IL-2Rα]/[cytokine production in the presence of the isotype control antibody]) × 100%. Depicted are mean and SEM of six independent experiments. Significant inhibition (P < .01) of cytokine production is indicated by (*).