Ploidy Distribution of Megakaryocytes Supported by Specified Factors in Serum-Free Culture
| Factors . | No. of Megakaryocytes . | Ploidy Class (%)4-150 . | Mean ± SD . | ||||
|---|---|---|---|---|---|---|---|
| . | Analyzed . | 2N . | 4N . | 8N . | 16N . | 32N . | . |
| IL-3 | 150 | 72.7 | 19.3 | 7.3 | 0.7 | 2.92 ± 1.97 | |
| SCF + IL-3 (NS) | 150 | 66.7 | 23.3 | 10.0 | 3.07 ± 1.84 | ||
| SCF + IL-6 + sIL-6R4-151 | 150 | 54.0 | 26.0 | 20.0 | 3.72 ± 2.30 | ||
| IL-3 + IL-6 + sIL-6R‡ | 200 | 48.5 | 29.0 | 12.5 | 10.0 | 4.63 ± 4.22 | |
| SCF + IL-3 + IL-6 + sIL-6Rρ | 200 | 44.0 | 18.5 | 23.5 | 8.5 | 5.5 | 6.62 ± 7.35 |
| TPOρ | 150 | 19.3 | 22.0 | 28.7 | 24.7 | 5.3 | 9.21 ± 7.45 |
| Factors . | No. of Megakaryocytes . | Ploidy Class (%)4-150 . | Mean ± SD . | ||||
|---|---|---|---|---|---|---|---|
| . | Analyzed . | 2N . | 4N . | 8N . | 16N . | 32N . | . |
| IL-3 | 150 | 72.7 | 19.3 | 7.3 | 0.7 | 2.92 ± 1.97 | |
| SCF + IL-3 (NS) | 150 | 66.7 | 23.3 | 10.0 | 3.07 ± 1.84 | ||
| SCF + IL-6 + sIL-6R4-151 | 150 | 54.0 | 26.0 | 20.0 | 3.72 ± 2.30 | ||
| IL-3 + IL-6 + sIL-6R‡ | 200 | 48.5 | 29.0 | 12.5 | 10.0 | 4.63 ± 4.22 | |
| SCF + IL-3 + IL-6 + sIL-6Rρ | 200 | 44.0 | 18.5 | 23.5 | 8.5 | 5.5 | 6.62 ± 7.35 |
| TPOρ | 150 | 19.3 | 22.0 | 28.7 | 24.7 | 5.3 | 9.21 ± 7.45 |
One hundred fifty or 200 megakaryocytes were analyzed for relative nuclear DNA content. Statistical significance was calculated by the χ2 test.
Abbreviation: NS, not significant.
Percentage of megakaryocytes in each ploidy class. Ploidy distribution was compared between IL-3 and the specified factors. The mean nuclear DNA content supported by the three signals was significantly less than that supported by TPO (P < .01). Granulocytes obtained from pure neutrophilic colonies formed in the cultures were used as the diploid standards.
P < .05.
P < .02.
ρ P < .01.