Pure and Mixed Megakaryocyte Colony Formation by PB-Derived CD34+IL-6R− Cells in Serum-Free Culture
| Factors . | Colony Types . | |||
|---|---|---|---|---|
| . | Meg . | M-Mix . | Others . | Total . |
| TPO | 140 | 0 | 0 | 140 |
| SCF | 0 | 0 | 5 | 5 |
| IL-3 | 35 | 24 | 131 | 190 |
| SCF + IL-3 | 38 | 32 | 227 | 297 |
| SCF + IL-6 + sIL-6R | 47 | 24 | 76 | 147 |
| IL-3 + IL-6 + sIL-6R | 20 | 57 | 332 | 409 |
| SCF + IL-3 + IL-6 + sIL-6R | 7 | 135 | 607 | 749 |
| Factors . | Colony Types . | |||
|---|---|---|---|---|
| . | Meg . | M-Mix . | Others . | Total . |
| TPO | 140 | 0 | 0 | 140 |
| SCF | 0 | 0 | 5 | 5 |
| IL-3 | 35 | 24 | 131 | 190 |
| SCF + IL-3 | 38 | 32 | 227 | 297 |
| SCF + IL-6 + sIL-6R | 47 | 24 | 76 | 147 |
| IL-3 + IL-6 + sIL-6R | 20 | 57 | 332 | 409 |
| SCF + IL-3 + IL-6 + sIL-6R | 7 | 135 | 607 | 749 |
Data represent the total number of colonies derived from 10 dishes each containing 200 CD34+IL-6R− cells.
Abbreviations: Meg, pure megakaryocyte colony; M-Mix, megakaryocyte-containing mixed colony.