Percentage of Cells Directly Sorted or After In Vitro Assays With the Leukemic Change by FISH or Cytogenetics (no. of colonies analyzed)
| Patient No. . | % Positive . | % Positive Cells . | % Positive Cells . |
|---|---|---|---|
| . | Nucleated Cells . | From CFU-blast . | From CFU in SC . |
| Control | |||
| 1 | 70 | 83 (20) | 96 (30) |
| 2 | 100 | ND | ND |
| 3 | 82 | 76 (10) | 69 (20) |
| 4 | ND | ND | 63 (14) |
| 5 | 73 | ND | 76 (5) |
| 9 | ND | ND | 60 (15) |
| 10 | ND | 65 (5) | 76 (5) |
| 14 | ND | 81 (10) | ND |
| 15 | ND | 81 (10) | 76 (20) |
| Thy-1+ cells | |||
| 3 | 89 | ND | 6 (20) |
| 5 | 81 | ND | ND |
| CD34+/Thy-1+ | |||
| 1 | 69 | 91 (5) | ND |
| 2 | 99 | ND | ND |
| 3 | 72 | 78 (10) | 22 (10) |
| 4 | ND | ND | 28 (10) |
| 5 | 75 | ND | 29 (5) |
| 15 | ND | 81 (5) | 53 (5) |
| CD34+/Thy-1− | |||
| 1 | ND | 74 (10) | ND |
| 4 | ND | ND | 70 (10) |
| 9 | ND | ND | 77 (30) |
| 10 | ND | ND | 68 (5) |
| 15 | ND | 80 (15) | 75 (20) |
| Patient No. . | % Positive . | % Positive Cells . | % Positive Cells . |
|---|---|---|---|
| . | Nucleated Cells . | From CFU-blast . | From CFU in SC . |
| Control | |||
| 1 | 70 | 83 (20) | 96 (30) |
| 2 | 100 | ND | ND |
| 3 | 82 | 76 (10) | 69 (20) |
| 4 | ND | ND | 63 (14) |
| 5 | 73 | ND | 76 (5) |
| 9 | ND | ND | 60 (15) |
| 10 | ND | 65 (5) | 76 (5) |
| 14 | ND | 81 (10) | ND |
| 15 | ND | 81 (10) | 76 (20) |
| Thy-1+ cells | |||
| 3 | 89 | ND | 6 (20) |
| 5 | 81 | ND | ND |
| CD34+/Thy-1+ | |||
| 1 | 69 | 91 (5) | ND |
| 2 | 99 | ND | ND |
| 3 | 72 | 78 (10) | 22 (10) |
| 4 | ND | ND | 28 (10) |
| 5 | 75 | ND | 29 (5) |
| 15 | ND | 81 (5) | 53 (5) |
| CD34+/Thy-1− | |||
| 1 | ND | 74 (10) | ND |
| 4 | ND | ND | 70 (10) |
| 9 | ND | ND | 77 (30) |
| 10 | ND | ND | 68 (5) |
| 15 | ND | 80 (15) | 75 (20) |
Abbreviations: CFU-blast, leukemic clusters (10-20 cells) plus colonies (>20 cells) plucked from methylcellulose; CFU in SC, CFU-blast derived from suspension cultures; ND, not determined.