Adsorption Studies of Multiple Quinine-Dependent Antibodies
| . | MFI . | ||||
|---|---|---|---|---|---|
| . | Platelets . | T Lymphocytes (CD3+) . | B Lymphocytes (CD19+) . | Neutrophils (CD13+) . | Neutrophils (CD33+) . |
| Patient serum | 1.3 | 3.5 | 12.2 | 13.0 | 14.3 |
| Patient serum + quinine | 112.4 | 14.6 | 66.1 | 417.9 | 365.2 |
| Patient serum absorbed with neutrophils + quinine | 115.5 | 15.0 | 67.3 | 18.4 | 21.3 |
| Patient serum absorbed with mononuclear cells + quinine | 105.5 | 3.6 | 8.1 | 346.0 | 410.3 |
| . | MFI . | ||||
|---|---|---|---|---|---|
| . | Platelets . | T Lymphocytes (CD3+) . | B Lymphocytes (CD19+) . | Neutrophils (CD13+) . | Neutrophils (CD33+) . |
| Patient serum | 1.3 | 3.5 | 12.2 | 13.0 | 14.3 |
| Patient serum + quinine | 112.4 | 14.6 | 66.1 | 417.9 | 365.2 |
| Patient serum absorbed with neutrophils + quinine | 115.5 | 15.0 | 67.3 | 18.4 | 21.3 |
| Patient serum absorbed with mononuclear cells + quinine | 105.5 | 3.6 | 8.1 | 346.0 | 410.3 |
The cell-bound IgG was analyzed by using flow cytometry. The results are expressed as median fluorescence intensity (MFI) and are the mean of 2 experiments.