Flow Cytometric Analysis of Multiple Quinine-Dependent Antibodies
. | MFI . | ||||
---|---|---|---|---|---|
. | Platelets . | T Lymphocytes (CD3+) . | B Lymphocytes (CD19+) . | Neutrophils (CD13+) . | Neutrophils (CD33+) . |
Normal serum | 3.8 ± 2.5 | 19.3 ± 10.8 | 3.7 ± 2.2 | 32.8 ± 15.7 | 29.2 ± 10.2 |
Normal serum + quinine | 3.9 ± 2.6 | 18.2 ± 7.9 | 3.7 ± 2.2 | 31.6 ± 14.3 | 29.9 ± 10.5 |
Patient serum | 5.8 ± 4.1 | 18.5 ± 8.2 | 3.8 ± 2.4 | 33.9 ± 16.9 | 30.4 ± 11.4 |
Patient serum + quinine | 204.7 ± 92.3* | 165.6 ± 95* | 28.1 ± 13.2* | 1159 ± 930* | 1300 ± 1011.2* |
. | MFI . | ||||
---|---|---|---|---|---|
. | Platelets . | T Lymphocytes (CD3+) . | B Lymphocytes (CD19+) . | Neutrophils (CD13+) . | Neutrophils (CD33+) . |
Normal serum | 3.8 ± 2.5 | 19.3 ± 10.8 | 3.7 ± 2.2 | 32.8 ± 15.7 | 29.2 ± 10.2 |
Normal serum + quinine | 3.9 ± 2.6 | 18.2 ± 7.9 | 3.7 ± 2.2 | 31.6 ± 14.3 | 29.9 ± 10.5 |
Patient serum | 5.8 ± 4.1 | 18.5 ± 8.2 | 3.8 ± 2.4 | 33.9 ± 16.9 | 30.4 ± 11.4 |
Patient serum + quinine | 204.7 ± 92.3* | 165.6 ± 95* | 28.1 ± 13.2* | 1159 ± 930* | 1300 ± 1011.2* |
The results (mean ± SD of 6 experiments) are expressed as median fluorescence intensity (MFI).
P < .01. Comparisons were made for patient serum in the presence and absence of quinine.