Binding of α-CXCR Antibodies to PMN Pretreated With IL-8
| IL-8 Pretreatment* . | % Specific Binding of Antibody† . | |
|---|---|---|
| . | α-CXCR–1 . | α-CXCR–2 . |
| 37°C | 12.0 ± 5.7‡ | 6.6 ± 4.7 |
| 4°C | 101.3 ± 1.5 | 98.1 ± 7.3 |
| IL-8 Pretreatment* . | % Specific Binding of Antibody† . | |
|---|---|---|
| . | α-CXCR–1 . | α-CXCR–2 . |
| 37°C | 12.0 ± 5.7‡ | 6.6 ± 4.7 |
| 4°C | 101.3 ± 1.5 | 98.1 ± 7.3 |
Neutrophils were pretreated with 2 μmol/L IL-8 at 37°C or 4°C or left untreated for 10 minutes.
Binding of α-CXCR–1 (MoAb RII115) and α-CXCR–2 (MoAb SE-2) as detected by staining with fluorescein-conjugated goat α-mouse IgG was recorded as median fluorescence intensity by flow cytometry. Unspecific fluorescence of PMN as determined in the absence of α-CXCR antibodies was subtracted (the proportion of unspecific binding was as depicted in Fig 2).
Data are given as percentage of controls that received no chemokine pretreatment and represent mean ± SD of three different experiments.