Ploidy Distribution of CD34-Derived Megakaryocytic Cells
| . | d 12 . | d 15 . | d 18 . | d 21 . |
|---|---|---|---|---|
| 2N | 22 | 18 | 37 | 54 |
| 4N | 24 | 21 | 33 | 37 |
| 8N | 20 | 18 | 22 | 8 |
| 16N | 18 | 25 | 7 | 1 |
| >16N | 16 | 18 | 1 | 0 |
| . | d 12 . | d 15 . | d 18 . | d 21 . |
|---|---|---|---|---|
| 2N | 22 | 18 | 37 | 54 |
| 4N | 24 | 21 | 33 | 37 |
| 8N | 20 | 18 | 22 | 8 |
| 16N | 18 | 25 | 7 | 1 |
| >16N | 16 | 18 | 1 | 0 |
The DNA content was evaluated by flow cytometry after PI staining at different time points of liquid cultures supplemented with 100 ng/mL of TPO. Data represent the means of three separate experiments, each performed in duplicate starting from a different CD34+ cell sample.