Primers Used for V Gene Amplification
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1 leader | CACCATGGACTGACCTGGA |
| VH2 leader | ATGGACATACTTTGTTCCAC |
| VH3 leader | CCATGGAGTTTGGGCTGAGC |
| VH4 leader | ATGAAACACCTGTGGTTCTT |
| VH5 leader | ATGGGGTCAACCGCCATCCT |
| VH6 leader | ATGTCTGTCTCCTTCCTCAT |
| FW3 | GACACGGC(C,T)(A,G)TGTATTACTG |
| JH | ACCTGAGGAGACGGTGACC |
| Cμ | GTCCATCCGCCCCAACCCTT |
| VK1 leader | ATGGACATGAGGGTCCCCGC |
| VK2 leader | ATGAGGCTCCCTGCTCAGCT |
| VK3 leader | ATGGAAACCCCAGC(G,T)CAGCT |
| VK4 leader | ATGGTGTTGCAGACCCAGGTC |
| VK5 leader | ATGGGGTCCCAGGTTCACCTC |
| VK6 leader | ATGTTGCCATCACAACTCATTG |
| JK124 | ACGTTTGAT(C,T)TCCA(C,G)CTTGGTC |
| JK3 | ACGTTTGATATCCACTTTGGTC |
| JK5 | ACGTTTAATCTCCAGTCGTGTC |
| CK | AGATGGCGGGAAGATGAAGAC |
| Name . | Sequence (5′-3′) . |
|---|---|
| VH1 leader | CACCATGGACTGACCTGGA |
| VH2 leader | ATGGACATACTTTGTTCCAC |
| VH3 leader | CCATGGAGTTTGGGCTGAGC |
| VH4 leader | ATGAAACACCTGTGGTTCTT |
| VH5 leader | ATGGGGTCAACCGCCATCCT |
| VH6 leader | ATGTCTGTCTCCTTCCTCAT |
| FW3 | GACACGGC(C,T)(A,G)TGTATTACTG |
| JH | ACCTGAGGAGACGGTGACC |
| Cμ | GTCCATCCGCCCCAACCCTT |
| VK1 leader | ATGGACATGAGGGTCCCCGC |
| VK2 leader | ATGAGGCTCCCTGCTCAGCT |
| VK3 leader | ATGGAAACCCCAGC(G,T)CAGCT |
| VK4 leader | ATGGTGTTGCAGACCCAGGTC |
| VK5 leader | ATGGGGTCCCAGGTTCACCTC |
| VK6 leader | ATGTTGCCATCACAACTCATTG |
| JK124 | ACGTTTGAT(C,T)TCCA(C,G)CTTGGTC |
| JK3 | ACGTTTGATATCCACTTTGGTC |
| JK5 | ACGTTTAATCTCCAGTCGTGTC |
| CK | AGATGGCGGGAAGATGAAGAC |