Effect of rD-mPGPtide on Reconstitution of Donor T and B Lymphocytes in the Haploidentical and MHC Class II-Disparate Strain Combinations
| Strain Combination . | Treatment . | Phenotype, Day 30; Spleen (% donor/host) . | Phenotype, Day 72; Spleen* (% donor/host) . | Phenotype, Day 72; . | |||
|---|---|---|---|---|---|---|---|
| . | . | T Cells . | B Cells . | T Cells . | B Cells . | LN* (% donor/host) . | |
| . | . | . | . | . | . | T Cells . | B Cells . |
| (B6D2)F1 → (B6CB)F1 (800 cGy) | PBS | 8/47 | 5/26 | 7/59 | 6/39 | 6/28 | |
| rD-mPGPtide | 32/11 | ND | 36/9 | 64/4 | 68/16 | 28/4 | |
| Anti-CD4 MoAb | 29/14 | 32/10 | 61/5 | 61/20 | 27/5 | ||
| bm12 → B6-Ly5.2 (750 cGy) | PBS | 9/22 | 10/44 | 4/25 | 2/57 | ||
| rD-mPGPtide | 28/11 | 41/11 | 14/10 | ND | 37/13 | ND | |
| Anti-CD4 MoAb | 21/9 | 56/6 | 15/8 | 31/14 | |||
| Strain Combination . | Treatment . | Phenotype, Day 30; Spleen (% donor/host) . | Phenotype, Day 72; Spleen* (% donor/host) . | Phenotype, Day 72; . | |||
|---|---|---|---|---|---|---|---|
| . | . | T Cells . | B Cells . | T Cells . | B Cells . | LN* (% donor/host) . | |
| . | . | . | . | . | . | T Cells . | B Cells . |
| (B6D2)F1 → (B6CB)F1 (800 cGy) | PBS | 8/47 | 5/26 | 7/59 | 6/39 | 6/28 | |
| rD-mPGPtide | 32/11 | ND | 36/9 | 64/4 | 68/16 | 28/4 | |
| Anti-CD4 MoAb | 29/14 | 32/10 | 61/5 | 61/20 | 27/5 | ||
| bm12 → B6-Ly5.2 (750 cGy) | PBS | 9/22 | 10/44 | 4/25 | 2/57 | ||
| rD-mPGPtide | 28/11 | 41/11 | 14/10 | ND | 37/13 | ND | |
| Anti-CD4 MoAb | 21/9 | 56/6 | 15/8 | 31/14 | |||
Data from one experiment representative of three separate experiments are presented as the percentage of positive cells, as analyzed by flow cytometry. Chimerism was determined using the following MoAb pairs: PE-anti-Thy1.2 (or B220) and FITC-anti-H2Kk, [(B6D2)F1 → (B6CB)F1 ]; PE-anti-Thy1.2 (or B220) and FITC-anti-CD45.1 (anti-Ly5.1) (bm12 → B6-Ly5.2). The mean of samples from five mice per group is presented for each individual treatment. The values in all rD-mPGPtide and anti-CD4 MoAb treatment groups were significantly different from those in the PBS control group, P ≤ .001.
Abbreviation: ND, not done.
Mice in the bm12 → B6-Ly5.2 strain combination were analyzed on day 60 after transplantation.