Overall Reduction of Colonies Formed by Spleen-Derived Hematopoietic Progenitor Cells in CD44-Deficient Mice
| . | . | PBS . | G-CSF . |
|---|---|---|---|
| Spleen (colonies/105 cells) | CD44+/− | 13.25 ± 2.75 | 203.50 ± 11.00 |
| CD44−/− | 7.25 ± 2.63 | 113.00 ± 12.40 | |
| (P = .02) | (P < .001) | ||
| Bone marrow (colonies/104 cells) | CD44+/− | 27.25 ± 4.92 | 22.75 ± 4.99 |
| CD44−/− | 30.75 ± 5.85 | 33.00 ± 9.90 | |
| (P = .395) | (P = .022) | ||
| Peripheral blood (colonies/105 cells) | CD44+/− | — | 10.82 ± 4.32 |
| CD44−/− | — | 4.16 ± 5.46 | |
| (P = .149) |
| . | . | PBS . | G-CSF . |
|---|---|---|---|
| Spleen (colonies/105 cells) | CD44+/− | 13.25 ± 2.75 | 203.50 ± 11.00 |
| CD44−/− | 7.25 ± 2.63 | 113.00 ± 12.40 | |
| (P = .02) | (P < .001) | ||
| Bone marrow (colonies/104 cells) | CD44+/− | 27.25 ± 4.92 | 22.75 ± 4.99 |
| CD44−/− | 30.75 ± 5.85 | 33.00 ± 9.90 | |
| (P = .395) | (P = .022) | ||
| Peripheral blood (colonies/105 cells) | CD44+/− | — | 10.82 ± 4.32 |
| CD44−/− | — | 4.16 ± 5.46 | |
| (P = .149) |
CD44 influences number/mobilization of hematopoietic progenitor cells. Mice were treated with G-CSF (125 μg/kg body weight) or PBS twice daily for 4 days. At day 7 spleen, bone marrow, and peripheral blood cells were collected. Colonies were grown in supplemented Iscove's Modified Dulbecco's Medium 1% methylcellulose. Colonies were counted at day 8 after plating. Values are presented as mean ± standard error for quadruplicate cultures. Significance was calculated by Student's t-test.
Abbreviations: PBS, phosphate buffered saline; G-CSF, granulocyte colony-stimulating factor.