Inhibition of CLL B-Cell and Normal B-Cell Proliferation by TGF-β
| . | Proliferation SAC/MLA (cpm)* . | Proliferation SAC/MLA + . | Percent Inhibition† . |
|---|---|---|---|
| . | . | TGF-β (cpm) . | . |
| CLL B-cell donor | |||
| CLL 1 | 4,384 | 2,910 | 34 |
| CLL 2 | 29,180 | 21,710 | 26 |
| CLL 3 | 9,392 | 7,581 | 19 |
| CLL 4 | 1,612 | 610 | 62 |
| CLL 5 | 8,118 | 1,774 | 78 |
| CLL 6 | 21,193 | 2,322 | 89 |
| CLL 7 | 18,662 | 8,895 | 52 |
| CLL 8 | 2,549 | 500 | 80 |
| CLL 9 | 2,294 | 884 | 61 |
| CLL 10 | 3,879 | 2,195 | 43 |
| CLL 11 | 3,379 | 3,297 | 2 |
| CLL 12 | 1,399 | 985 | 30 |
| CLL 13 | 2,732 | 1,806 | 34 |
| CLL 14 | 2,178 | 1,237 | 43 |
| CLL 15 | 1,729 | 419 | 76 |
| CLL 16 | 18,135 | 2,422 | 87 |
| CLL 17 | 1,327 | 865 | 35 |
| CLL 18 | 2,345 | 1,016 | 57 |
| CLL 19 | 1,159 | 370 | 68 |
| CLL 20 | 6,483 | 5,575 | 14 |
| Normal B-cell donor | |||
| N 1 | 13,262 | 2,819 | 79 |
| N 2 | 22,467 | 9,021 | 60 |
| N 3 | 31,713 | 8,043 | 75 |
| N 4 | 36,000 | 10,440 | 71 |
| N 5 | 51,623 | 9,292 | 82 |
| N 6 | 42,000 | 30,240 | 28 |
| N 7 | 31,456 | 9,122 | 71 |
| N 8 | 24,398 | 7,319 | 70 |
| N 9 | 38,988 | 11,306 | 71 |
| N 10 | 21,786 | 9,926 | 54 |
| N 11 | 40,398 | 18,866 | 53 |
| . | Proliferation SAC/MLA (cpm)* . | Proliferation SAC/MLA + . | Percent Inhibition† . |
|---|---|---|---|
| . | . | TGF-β (cpm) . | . |
| CLL B-cell donor | |||
| CLL 1 | 4,384 | 2,910 | 34 |
| CLL 2 | 29,180 | 21,710 | 26 |
| CLL 3 | 9,392 | 7,581 | 19 |
| CLL 4 | 1,612 | 610 | 62 |
| CLL 5 | 8,118 | 1,774 | 78 |
| CLL 6 | 21,193 | 2,322 | 89 |
| CLL 7 | 18,662 | 8,895 | 52 |
| CLL 8 | 2,549 | 500 | 80 |
| CLL 9 | 2,294 | 884 | 61 |
| CLL 10 | 3,879 | 2,195 | 43 |
| CLL 11 | 3,379 | 3,297 | 2 |
| CLL 12 | 1,399 | 985 | 30 |
| CLL 13 | 2,732 | 1,806 | 34 |
| CLL 14 | 2,178 | 1,237 | 43 |
| CLL 15 | 1,729 | 419 | 76 |
| CLL 16 | 18,135 | 2,422 | 87 |
| CLL 17 | 1,327 | 865 | 35 |
| CLL 18 | 2,345 | 1,016 | 57 |
| CLL 19 | 1,159 | 370 | 68 |
| CLL 20 | 6,483 | 5,575 | 14 |
| Normal B-cell donor | |||
| N 1 | 13,262 | 2,819 | 79 |
| N 2 | 22,467 | 9,021 | 60 |
| N 3 | 31,713 | 8,043 | 75 |
| N 4 | 36,000 | 10,440 | 71 |
| N 5 | 51,623 | 9,292 | 82 |
| N 6 | 42,000 | 30,240 | 28 |
| N 7 | 31,456 | 9,122 | 71 |
| N 8 | 24,398 | 7,319 | 70 |
| N 9 | 38,988 | 11,306 | 71 |
| N 10 | 21,786 | 9,926 | 54 |
| N 11 | 40,398 | 18,866 | 53 |
Data shown were maximum proliferation: CLL B cells (3- to 5-day culture), normal B cells (3 day culture); Sixteen hours before harvest, 2 μCi 3H-thymidine was added to each well. Proliferation was measured by 3H-thymidine incorporation in cpm.
Percent inhibition of proliferation was calculated as: {[Proliferation SAC/MLA − Proliferation SAC/MLA/TGF-β] / [Proliferation SAC/MLA]} × 100.