Retroviral Transduction of CD34+CD38− Cells Is Enhanced by FLT3L
| HGF . | Experiment 1 . | Experiment 2 . | Experiment 3 . |
|---|---|---|---|
| Control | 0 | 0 | 0 |
| IL-3, IL-6, SCF | ND | 2 (5/250) | ND |
| 4 HGF | 8 (11/143) | 5 (13/250) | 11 (16/150) |
| 4 HGF + FLT3L | 17 (27/173) | 14 (36/260) | 22 (41/168) |
| HGF . | Experiment 1 . | Experiment 2 . | Experiment 3 . |
|---|---|---|---|
| Control | 0 | 0 | 0 |
| IL-3, IL-6, SCF | ND | 2 (5/250) | ND |
| 4 HGF | 8 (11/143) | 5 (13/250) | 11 (16/150) |
| 4 HGF + FLT3L | 17 (27/173) | 14 (36/260) | 22 (41/168) |
Abbreviation: ND, not determined.
Three different sources of BM CD34+CD38− cells were cultured with LAPSN retrovirus without HGF (control), with IL-3 + IL-6 + SCF, 4 HGF and 4 HGF + FLT3L. Cells were examined for presence of alkaline phosphatase and the percentage of positive (positive cells/total cells examined) expressed as a proportion of total cells. A significantly (P = .0047) greater proportion of the CD34+CD38− cells were transduced when cultured with 4 HGF + FLT3L.