Activated γδ T Cells From Pure Background β0 Donors Facilitate Alloengraftment and Enhance Hematopoietic Reconstitution
| Group . | No. of Mice . | Donor γδ T Cells Added . | (%) Donor T Cells7-150 . | (Mean) . | (%) (H-2b) Cells . | (Mean) . | WBC . | HCT . | Platelets . |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | (per mm3) . | . | (×1,000) . |
| I | 3 | None | 1, 1, 3 | (2) | 5, 7, 9 | (7) | 4.7 ± 1.2 | 40 ± 9.7 | 474 ± 159 |
| II | 5 | 40 × 106 | 2, 62, 24, 47, 1 | (27) | 7, 91, 54, 85, 5 | (48) | 6.9 ± 3.7 | 46 ± 5.2 | 765 ± 484 |
| III | 5 | 80 × 106 | 69, 58, 55, 3, 54 | (48†) | 91, 87, 89, 5, 85 | (71†) | 8.6 ± 1.67-151 | 46 ± 1.6 | 1,089 ± 1377-151 |
| Group . | No. of Mice . | Donor γδ T Cells Added . | (%) Donor T Cells7-150 . | (Mean) . | (%) (H-2b) Cells . | (Mean) . | WBC . | HCT . | Platelets . |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | (per mm3) . | . | (×1,000) . |
| I | 3 | None | 1, 1, 3 | (2) | 5, 7, 9 | (7) | 4.7 ± 1.2 | 40 ± 9.7 | 474 ± 159 |
| II | 5 | 40 × 106 | 2, 62, 24, 47, 1 | (27) | 7, 91, 54, 85, 5 | (48) | 6.9 ± 3.7 | 46 ± 5.2 | 765 ± 484 |
| III | 5 | 80 × 106 | 69, 58, 55, 3, 54 | (48†) | 91, 87, 89, 5, 85 | (71†) | 8.6 ± 1.67-151 | 46 ± 1.6 | 1,089 ± 1377-151 |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6 marrow cells with or without the indicated number of activated γδ T cells from β 0 donors (pure B6 background). Animals were evaluated 35 to 37 days posttransplant. Donor T cells were defined as Thy 1.2+/CD3+ and donor cells as H-2b+. PB counts are reported as the mean ± 1 SD. Normal ranges for blood counts for comparison were determined by obtaining blood samples from normal nontransplanted C57BL/6 mice (n = 4). These measurements were as follows: WBC 10.3 ± 1.5, HCT 49 ± 1.1, and platelets 1,070 ± 62.
Numbers represent data for individual mice.
P ≤ .03 relative to group I.