Facilitation of Long-Term Alloengraftment (<100 Day) by γδ T Cells
| Group . | No. of Mice . | Donor αβ T Cells Added . | Donor γδ T Cells Added . | (%) Donor T Cells6-150 . | (%) Donor (H-2b) Cells6-151 . | Splenic B Cells (×10−6) . | Spleen Size . | Day 100 . | |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | (×10−6) . | No. Mice Surviving . | No. Mice Engrafting . |
| I | 6 | None | None | 49, 3, 1, 1, 3 | 94, 6, 3, 3, 3 | 77, 26, 24, 28, 23 | 111, 45, 56, 65, 54 | 5/6 | 1/6 |
| II | 4 | 1 × 105 | None | 83, 2 | 96, 4 | 29, 23 | 52, 34 | 2/4 | 1/4 |
| III | 4 | 5 × 105 | None | 87, 82, 90, 88 | 99, 98, 99, 98 | 21, 41, 8, 16 | 56, 78, 19, 39 | 4/4 | 4/4 |
| IV | 3 | 1 × 106 | None | 97, 80, 90 | 94, 99, 95 | 0, 5, 1 | 1, 16, 2 | 3/3 | 3/3 |
| V | 5 | None | 20 × 106 | 1, 0, 86‡, 0 | 4, 3, 98, 10 | 18, 7, 15, 3 | NA | 3/5 | 1/5 |
| VI | 3 | None | 160 × 106 | 83, 86, 84 | 98, 97, 96 | 120, 63, 36 | 204, 147, 73 | 3/3 | 3/3 |
| VII | 3 | 2.5 × 104 | None | 3, 3, 89 | 3, 2, 98 | 18, 14, 58 | 44, 31, 144 | 3/3 | 1/3 |
| VIII | 4 | 2.5 × 104 | 160 × 106 | 95, 95, 96, 94 | 100, 100, 100, 100 | 58, 87, 10, 54 | 154, 175, 39, 117 | 4/4 | 4/4 |
| Group . | No. of Mice . | Donor αβ T Cells Added . | Donor γδ T Cells Added . | (%) Donor T Cells6-150 . | (%) Donor (H-2b) Cells6-151 . | Splenic B Cells (×10−6) . | Spleen Size . | Day 100 . | |
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | (×10−6) . | No. Mice Surviving . | No. Mice Engrafting . |
| I | 6 | None | None | 49, 3, 1, 1, 3 | 94, 6, 3, 3, 3 | 77, 26, 24, 28, 23 | 111, 45, 56, 65, 54 | 5/6 | 1/6 |
| II | 4 | 1 × 105 | None | 83, 2 | 96, 4 | 29, 23 | 52, 34 | 2/4 | 1/4 |
| III | 4 | 5 × 105 | None | 87, 82, 90, 88 | 99, 98, 99, 98 | 21, 41, 8, 16 | 56, 78, 19, 39 | 4/4 | 4/4 |
| IV | 3 | 1 × 106 | None | 97, 80, 90 | 94, 99, 95 | 0, 5, 1 | 1, 16, 2 | 3/3 | 3/3 |
| V | 5 | None | 20 × 106 | 1, 0, 86‡, 0 | 4, 3, 98, 10 | 18, 7, 15, 3 | NA | 3/5 | 1/5 |
| VI | 3 | None | 160 × 106 | 83, 86, 84 | 98, 97, 96 | 120, 63, 36 | 204, 147, 73 | 3/3 | 3/3 |
| VII | 3 | 2.5 × 104 | None | 3, 3, 89 | 3, 2, 98 | 18, 14, 58 | 44, 31, 144 | 3/3 | 1/3 |
| VIII | 4 | 2.5 × 104 | 160 × 106 | 95, 95, 96, 94 | 100, 100, 100, 100 | 58, 87, 10, 54 | 154, 175, 39, 117 | 4/4 | 4/4 |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6129 BM with or without the indicated number of naive αβ, naive γδ, or activated γδ T cells. Naive αβ T cells were procured from normal B6129 mice. Activated γδ T cells (groups VI and VIII) were obtained from spleens of β 0 donors on a B6129 background, whereas naive γδ T cells (group V) were obtained from β 0 donors on a pure B6 background. Animals were sacrificed 99 to 103 days posttransplant and assessed for overall spleen size, B-cell reconstitution, and the extent of overall donor and T-cell chimerism.
Key statistical comparisons: groups III, IV, VI, and VIII v I, P < .002; VI v VIII, P < .0001.
Groups III, IV, VI, and VIII v I, P < .02; VI v VIII, P < .05.
One animal in this group was evaluated at day 86 immediately antemortem.