Graft Enhancing Effect of Activated γδ T Cells
| Group . | No. of Mice . | Donor γδ T . | (%) Donor T Cells . | (Mean) . | No. of Splenic B Cells (×10−6) . | (Mean) . | (%) Donor (H-2b) Cells . | (Mean) . |
|---|---|---|---|---|---|---|---|---|
| . | . | Cells Added . | . | . | . | . | . | . |
| I | 12 | None | 2, 3, 2, 3, 3, 4, 6, 3, 4, 3, 2, 2 | (3) | 1, 8, 5, 0, 1, 1, 1, 0, 0, 1, 2, 0 | (1) | 4, 5, 2, 27, 5, 2, 8, 5, 5‡ | (7) |
| II | 5 | 5 × 106 | 7, 25, 4, 2, 16 | (11) | 0, 23, 2, 2, 1 | (6) | ND | |
| III | 5 | 10 × 106 | 5, 9, 8, 3, 3 | (6) | 4, 1, 10, 0, 1 | (3) | ND | |
| IV | 5 | 20 × 106 | 2, 3, 1, 5, 42 | (11) | 17, 0, 2, 1, 22 | (8) | ND | |
| V | 5 | 40 × 106 | 45, 25, 40, 5, 48 | (33*) | 36, 4, 28, 1, 8 | (15) | ND | |
| VI | 5 | 80 × 106 | 37, 37, 45, 4, 2 | (25) | 20, 44, 6, 1, 1 | (14) | 76, 85, 74, 11, 5 | (50) |
| VII | 4 | 160 × 106 | 57, 53, 20, 53 | (46*) | 49, 39, 1, 36 | (31) | 90, 88, 43, 90 | (78†) |
| VIII | 7 | 240 × 106 | 72, 69, 67, 64, 29, 0, 62 | (52*) | 52, 68, 61, 50, 4, 0, 28 | (38) | 94, 93, 92, 91, 82, 3, 91 | (78†) |
| Group . | No. of Mice . | Donor γδ T . | (%) Donor T Cells . | (Mean) . | No. of Splenic B Cells (×10−6) . | (Mean) . | (%) Donor (H-2b) Cells . | (Mean) . |
|---|---|---|---|---|---|---|---|---|
| . | . | Cells Added . | . | . | . | . | . | . |
| I | 12 | None | 2, 3, 2, 3, 3, 4, 6, 3, 4, 3, 2, 2 | (3) | 1, 8, 5, 0, 1, 1, 1, 0, 0, 1, 2, 0 | (1) | 4, 5, 2, 27, 5, 2, 8, 5, 5‡ | (7) |
| II | 5 | 5 × 106 | 7, 25, 4, 2, 16 | (11) | 0, 23, 2, 2, 1 | (6) | ND | |
| III | 5 | 10 × 106 | 5, 9, 8, 3, 3 | (6) | 4, 1, 10, 0, 1 | (3) | ND | |
| IV | 5 | 20 × 106 | 2, 3, 1, 5, 42 | (11) | 17, 0, 2, 1, 22 | (8) | ND | |
| V | 5 | 40 × 106 | 45, 25, 40, 5, 48 | (33*) | 36, 4, 28, 1, 8 | (15) | ND | |
| VI | 5 | 80 × 106 | 37, 37, 45, 4, 2 | (25) | 20, 44, 6, 1, 1 | (14) | 76, 85, 74, 11, 5 | (50) |
| VII | 4 | 160 × 106 | 57, 53, 20, 53 | (46*) | 49, 39, 1, 36 | (31) | 90, 88, 43, 90 | (78†) |
| VIII | 7 | 240 × 106 | 72, 69, 67, 64, 29, 0, 62 | (52*) | 52, 68, 61, 50, 4, 0, 28 | (38) | 94, 93, 92, 91, 82, 3, 91 | (78†) |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6129 marrow cells with or without the indicated number of activated γδ T cells. Activated γδ T cells were obtained from β0 donor animals (B6129 background) as described in Materials and Methods. Animals transplanted with 160 × 106 γδ T cells received two intravenous injections of 80 × 106 cells 1 hour apart. Mice transplanted with 240 × 106 γδ T cells received an additional injection of 80 × 106 cells 1 day posttransplant. All mice were analyzed 29 to 34 days after transplant.
P < .02 (Relative to group I).
P ≤ .002 (Relative to group I).
Only 9 of 12 mice in this group were tested.