Facilitation of Alloengraftment by Naive γδ T Cells
| Group . | No. of Mice . | Donor γδ T Cells Added . | (%) Donor T Cells3-150 . | (Mean) . | No. of Splenic B Cells (×10−6)3-150 . | (Mean) . |
|---|---|---|---|---|---|---|
| I | 18 | None | Mean 6 ± 5 | Mean 2 ± 3 | ||
| II | 5 | 1.3 × 106 | 3, 2, 1, 1 | (3) | 1, 1, 1, 0, 0 | (1) |
| III | 4 | 2.8 × 106 | 3, 3, 11, 2 | (5) | 1, 1, 1, 0 | (1) |
| IV | 4 | 5 × 106 | 12, 30, 45, 4 | (23) | 1, 20, 14, 1 | (9) |
| V | 7 | 10 × 106 | 62, 67, 66, 63, 6, 4, 28 | (42†) | 38, 40, 38, 12, 4, 1, 49 | (26) |
| VI | 7 | 20 × 106 | 43, 74, 79, 66, 1, 31, 45 | (48‡) | 3, 44, 18, 44, 1, 9, 32 | (22) |
| Group . | No. of Mice . | Donor γδ T Cells Added . | (%) Donor T Cells3-150 . | (Mean) . | No. of Splenic B Cells (×10−6)3-150 . | (Mean) . |
|---|---|---|---|---|---|---|
| I | 18 | None | Mean 6 ± 5 | Mean 2 ± 3 | ||
| II | 5 | 1.3 × 106 | 3, 2, 1, 1 | (3) | 1, 1, 1, 0, 0 | (1) |
| III | 4 | 2.8 × 106 | 3, 3, 11, 2 | (5) | 1, 1, 1, 0 | (1) |
| IV | 4 | 5 × 106 | 12, 30, 45, 4 | (23) | 1, 20, 14, 1 | (9) |
| V | 7 | 10 × 106 | 62, 67, 66, 63, 6, 4, 28 | (42†) | 38, 40, 38, 12, 4, 1, 49 | (26) |
| VI | 7 | 20 × 106 | 43, 74, 79, 66, 1, 31, 45 | (48‡) | 3, 44, 18, 44, 1, 9, 32 | (22) |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6129 marrow cells with or without the indicated number of γδ T cell enriched spleen cells (obtained from β0 donors on a B6129 background). The average number of γδ+ T cells in the splenic inocula after nylon wool depletion was 53%. The number of γδ T cells administered to recipients was quantitated by multiplying the percent of total cells which stained positively for Thy 1.2 and 3A10 antibodies by the total spleen cell dose. Donor T cells in the spleen were defined as Thy 1.2+/Lyt2+, Thy 1.2+/L3T4+, and Thy 1.2+/γδ TCR+. All mice in these experiments were evaluated 26 to 35 days posttransplant, except for the first four mice in group VI that were tested 59 to 61 days after transplant.
Numbers represent data for individual mice.
P < .02 (Relative to group I).
P < .007 (Relative to group I).