Alloengraftment Is Dependent on Mature Donor T Cells in The Marrow Graft
| Group . | No. of Mice . | Donor αβ T Cells Added . | (%) Donor T Cells* . | (Mean) . | No. of Splenic B Cells (×10−6)* . | (Mean) . |
|---|---|---|---|---|---|---|
| I | 11 | None | 1, 1, 1, 2, 3, 4, 1, 2, 2, 11, 1 | (3) | 2, 12, 0, 1, 3, 1, 1, 0, 0, 6, 1 | (2) |
| II | 4 | 1 × 104 | 3, 2, 5, 1 | (3) | 1, 1, 1, 1 | (1) |
| III | 7 | 5 × 104 | 79, 8, 0, 3, 57, 1, 1 | (21) | 17, 0, 0, 1, 37, 1, 0 | (8) |
| IV | 5 | 1 × 105 | 85, 90, 89, 69, 1 | (67†) | 32, 12, 24, 33, 2 | (21) |
| V | 4 | 5 × 105 | 90, 94, 62, 90 | (84†) | 14, 15, 1, 39 | (17) |
| VI | 4 | 1 × 106 | 93, 82, 88, 91 | (88†) | 40, 24, 24, 32 | (30) |
| VII | 4 | 2.3 × 106 | 96, 93, 95, 95 | (95†) | 0, 10, 2, 3 | (4) |
| Group . | No. of Mice . | Donor αβ T Cells Added . | (%) Donor T Cells* . | (Mean) . | No. of Splenic B Cells (×10−6)* . | (Mean) . |
|---|---|---|---|---|---|---|
| I | 11 | None | 1, 1, 1, 2, 3, 4, 1, 2, 2, 11, 1 | (3) | 2, 12, 0, 1, 3, 1, 1, 0, 0, 6, 1 | (2) |
| II | 4 | 1 × 104 | 3, 2, 5, 1 | (3) | 1, 1, 1, 1 | (1) |
| III | 7 | 5 × 104 | 79, 8, 0, 3, 57, 1, 1 | (21) | 17, 0, 0, 1, 37, 1, 0 | (8) |
| IV | 5 | 1 × 105 | 85, 90, 89, 69, 1 | (67†) | 32, 12, 24, 33, 2 | (21) |
| V | 4 | 5 × 105 | 90, 94, 62, 90 | (84†) | 14, 15, 1, 39 | (17) |
| VI | 4 | 1 × 106 | 93, 82, 88, 91 | (88†) | 40, 24, 24, 32 | (30) |
| VII | 4 | 2.3 × 106 | 96, 93, 95, 95 | (95†) | 0, 10, 2, 3 | (4) |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6129 marrow cells with or without the indicated number of αβ T cells. Enriched populations of αβ T cells were obtained by nylon wool depletion of spleen cells from B6129 donors. The average number of αβ+ T cells in the splenic inocula after nylon wool depletion was 86%. The number of αβ T cells administered to recipients was quantitated by multiplying the percent of total cells that stained positively with Thy 1.2 and H57-597 antibodies by the total spleen cell dose. Mice were sacrificed at 26 to 35 days posttransplant and chimerism in the spleen was assessed. Donor T cells were defined as Thy 1.2+/L3T4+ and Thy 1.2+/Lyt2+.
Numbers represent data for individual mice.
P < .02 relative to group I (no added T cells).