Donor-Type Engraftment of Murine Near-Term FNPB Cells in Sublethally Irradiated Adult Recipient Animals
| Time After Transplant . | Animal . | Peripheral Blood . | BM Colonies PCR . | |
|---|---|---|---|---|
| . | . | PCR/Hybridization (2 to 4 mo) . | FISH . | (>6 mo) . |
| . | . | . | (4 to 6 mo) . | . |
| Group 1 | 1 | pos | 3.7% | |
| Donor cells: fresh | 2 | pos | 2.0% | |
| Survivors: 5 of 7 | 3 | pos | 11.0% | |
| 4 | pos | 26.0% | ||
| 5 | pos | 6.3% | pos | |
| Group 2 | 1 | pos | 2.4% | |
| Donor cells: ex vivo culture | 2 | pos | 2.4% | |
| Survivors: 3 of 8* | 3 | pos | ND | pos |
| Time After Transplant . | Animal . | Peripheral Blood . | BM Colonies PCR . | |
|---|---|---|---|---|
| . | . | PCR/Hybridization (2 to 4 mo) . | FISH . | (>6 mo) . |
| . | . | . | (4 to 6 mo) . | . |
| Group 1 | 1 | pos | 3.7% | |
| Donor cells: fresh | 2 | pos | 2.0% | |
| Survivors: 5 of 7 | 3 | pos | 11.0% | |
| 4 | pos | 26.0% | ||
| 5 | pos | 6.3% | pos | |
| Group 2 | 1 | pos | 2.4% | |
| Donor cells: ex vivo culture | 2 | pos | 2.4% | |
| Survivors: 3 of 8* | 3 | pos | ND | pos |
Sublethally irradiated adult mice were transplanted with murine near-term FNPB cells, either fresh-untreated or after ex vivo culture with hematopoietic growth factors. Donor cells were genetically marked with the human MDR1 gene. Engraftment was tested by PCR amplification of the gene-marker in the peripheral blood of the survivors and by FISH analysis of their peripheral leukocytes. BM-derived colonies from two recipients were evaluated by PCR for the presence of the transgene.
Abbreviations: ND, not determined; pos, positive.
Spleen and bone marrow from 2 additional mice that died on day 8 posttransplant were positive for the MDR1 transgene by PCR analysis.