Summary of Additional Parameters Tested in HSA-Deficient Mice
| Parameter . | HSA-Positive Mice . | HSA-Deficient Mice . | ||
|---|---|---|---|---|
| . | 2 weeks old . | 9-12 weeks old . | 2 weeks old . | 9-12 weeks old . |
| Cellularity | ||||
| BM (total*) | 1.7 × 107 ± 0.3 (10) | 2.1 × 107 ± 0.6 (10) | 1.5 × 107 ± 0.3 (6) | 2.4 × 107 ± 0.9 (8) |
| Spleen (total) | 4.5 × 107 ± 2 (10) | 1.8 × 108 ± 0.2 (4) | 5.1 × 107 ± 2.5 (6) | 2.0 × 108 ± 0.3 (4) |
| B cells | 56% ± 8 (8) | 63% ± 4 (4) | 46% ± 4 (3) | 56% ± 9 (4) |
| T cells | 29% ± 6 (8) | 28% ± 5 (4) | 31% ± 3 (3) | 30% ± 10 (4) |
| Gr1+ cells | 7% ± 1 (8) | ND | 8% ± 0.5 (3) | ND |
| Thymus | 2.2 × 108 ± 1.5 (8) | 1.3 × 108 ± 0.7 (8) | 2.5 × 108 ± 0.4 (3) | 1.5 × 108 ± 0.4 (4) |
| Erythrocytes cell number (×106/μL) | 8.1 ± 0.4 | 7.7 ± 0.2 | ||
| MCV (fL) | 48 ± 1 | 50 ± 1 | ||
| MCH (pg) | 16.1 ± 0.4 | 17.9 ± 0.1 | ||
| Hematocrit (%) | 38.9 ± 2.2 | 38.8 ± 1.8 | ||
| Reticulocytes (×103/μL) | 22.2 ± 4.8 | 17.0 ± 2.2 | ||
| Leukocytes (×103/μL) | 6.0 ± 1.5 | 4.9 ± 0.8 | ||
| Thrombocytes (×103/μL) | 708 ± 82 | 742 ± 162 | ||
| Monocytes (% leukocytes) | 3.2 ± 1.6 | 2.8 ± 0.8 | ||
| Segmented leukocytes (% leukocytes) | 7.2 ± 3.3 | 12.4 ± 2.7 | ||
| B1 (Ly-1+) B cells in peritoneum | 10% of total | 7% of total | ||
| Serum isotype levels for IgM, IgG1, IgG2b, IgG3, IgA in preimmune mice | No differences | |||
| Immunization | ||||
| TNP-Ficoll 1° | No difference | |||
| TNP-Ova, primary response | No difference | |||
| TNP-Ova, secondary response | No difference | |||
| Infection | ||||
| Listeria (liver titer) 5 days after infection | 2 × 105 ± 2 (5) | 4 × 105 ± 4 (4) | ||
| Borrelia | Up to 71 days after infection, no clinical signs of arthritis in either group (3) Borrelia could be isolated from skin biopsies of all animals at day 71. | |||
| No difference in inflammatory damage as judged by histological sections of lymph nodes, spleen, thymus, heart, liver, kidney, brain or knee joint. | ||||
| P chabaudi chabaudi (malaria) | Higher titers of infected erythrocytes in HSA-deficient mice but mice were able to clear infection and are immune to a second infection. | |||
| Parameter . | HSA-Positive Mice . | HSA-Deficient Mice . | ||
|---|---|---|---|---|
| . | 2 weeks old . | 9-12 weeks old . | 2 weeks old . | 9-12 weeks old . |
| Cellularity | ||||
| BM (total*) | 1.7 × 107 ± 0.3 (10) | 2.1 × 107 ± 0.6 (10) | 1.5 × 107 ± 0.3 (6) | 2.4 × 107 ± 0.9 (8) |
| Spleen (total) | 4.5 × 107 ± 2 (10) | 1.8 × 108 ± 0.2 (4) | 5.1 × 107 ± 2.5 (6) | 2.0 × 108 ± 0.3 (4) |
| B cells | 56% ± 8 (8) | 63% ± 4 (4) | 46% ± 4 (3) | 56% ± 9 (4) |
| T cells | 29% ± 6 (8) | 28% ± 5 (4) | 31% ± 3 (3) | 30% ± 10 (4) |
| Gr1+ cells | 7% ± 1 (8) | ND | 8% ± 0.5 (3) | ND |
| Thymus | 2.2 × 108 ± 1.5 (8) | 1.3 × 108 ± 0.7 (8) | 2.5 × 108 ± 0.4 (3) | 1.5 × 108 ± 0.4 (4) |
| Erythrocytes cell number (×106/μL) | 8.1 ± 0.4 | 7.7 ± 0.2 | ||
| MCV (fL) | 48 ± 1 | 50 ± 1 | ||
| MCH (pg) | 16.1 ± 0.4 | 17.9 ± 0.1 | ||
| Hematocrit (%) | 38.9 ± 2.2 | 38.8 ± 1.8 | ||
| Reticulocytes (×103/μL) | 22.2 ± 4.8 | 17.0 ± 2.2 | ||
| Leukocytes (×103/μL) | 6.0 ± 1.5 | 4.9 ± 0.8 | ||
| Thrombocytes (×103/μL) | 708 ± 82 | 742 ± 162 | ||
| Monocytes (% leukocytes) | 3.2 ± 1.6 | 2.8 ± 0.8 | ||
| Segmented leukocytes (% leukocytes) | 7.2 ± 3.3 | 12.4 ± 2.7 | ||
| B1 (Ly-1+) B cells in peritoneum | 10% of total | 7% of total | ||
| Serum isotype levels for IgM, IgG1, IgG2b, IgG3, IgA in preimmune mice | No differences | |||
| Immunization | ||||
| TNP-Ficoll 1° | No difference | |||
| TNP-Ova, primary response | No difference | |||
| TNP-Ova, secondary response | No difference | |||
| Infection | ||||
| Listeria (liver titer) 5 days after infection | 2 × 105 ± 2 (5) | 4 × 105 ± 4 (4) | ||
| Borrelia | Up to 71 days after infection, no clinical signs of arthritis in either group (3) Borrelia could be isolated from skin biopsies of all animals at day 71. | |||
| No difference in inflammatory damage as judged by histological sections of lymph nodes, spleen, thymus, heart, liver, kidney, brain or knee joint. | ||||
| P chabaudi chabaudi (malaria) | Higher titers of infected erythrocytes in HSA-deficient mice but mice were able to clear infection and are immune to a second infection. | |||
All comparisons were in age- and sex-matched C57BL/6 mice. The number of mice used is given in parentheses.
Abbreviation: ND, not done.
Total cells from two femurs.