Effect of C2 Ceramides and Sphingoid Bases on PEt and PA Formation During EIgG-Induced Phagocytosis
| Compound* . | Phosphatidylethanol (dpm/mg protein)† . | Phosphatidic Acid (dpm/mg protein)† . |
|---|---|---|
| Control | 13,347 ± 2,018 | 5,544 ± 582 |
| N-acetylsphingosine | 2,511 ± 661‡ | 2,978 ± 533‡ |
| N-acetyldihydrosphingosine | 14,769 ± 1,416 | 8,057 ± 1,680 |
| N-acetylphytosphingosine | 2,851 ± 466‡ | 3,880 ± 860ρ |
| Sphingosine | 49,392 ± 14,706ρ | 26,028 ± 7,668‡ |
| Dihydrosphingosine | 34,026 ± 5,258ρ | 19,713 ± 5,990ρ |
| Phytosphingosine | 35,649 ± 7,129‡ | 22,036 ± 3,491‡ |
| Compound* . | Phosphatidylethanol (dpm/mg protein)† . | Phosphatidic Acid (dpm/mg protein)† . |
|---|---|---|
| Control | 13,347 ± 2,018 | 5,544 ± 582 |
| N-acetylsphingosine | 2,511 ± 661‡ | 2,978 ± 533‡ |
| N-acetyldihydrosphingosine | 14,769 ± 1,416 | 8,057 ± 1,680 |
| N-acetylphytosphingosine | 2,851 ± 466‡ | 3,880 ± 860ρ |
| Sphingosine | 49,392 ± 14,706ρ | 26,028 ± 7,668‡ |
| Dihydrosphingosine | 34,026 ± 5,258ρ | 19,713 ± 5,990ρ |
| Phytosphingosine | 35,649 ± 7,129‡ | 22,036 ± 3,491‡ |
Values represent the mean ± SD for three experiments.
The final concentration of each compound tested was 10 μmol/L.
Phosphatidylethanol and phosphatidic acid were determined 30 minutes after addition of EIgG.
P < .01, significantly different from control.
ρ P < .05, significantly different from control.