Restriction Enzymes Used to Detect Mutations in the PK-L/R Gene
| Mutation . | Exon . | Recognition Sequence . | Restriction Enzyme for Normal Site . | Restriction Enzyme for Mutated Site . |
|---|---|---|---|---|
| 101-1G-A | n: gGGCCA | Sau96I: GGNCC | Hae I: WGGCCW | |
| m: aGGCCA | ||||
| 628delGT | 6 | n: CCGGGTCG | ScrFI: CCNGG | |
| m: CCGGCG | Nci I; Bcn I: CCSGG | |||
| G787A | 7 | n: CCCGGG | Sma I; Xma I: CCCGGG | EcoRII: CCWGG |
| m: CCCAGG | Msp I; HpaII: CCGG | |||
| G994A | 8 | n: GACGGC | No restriction site found | No restriction site found |
| m: GACAGC | ||||
| G1006T | 8 | n: TGGCAC | MaeIII: GTNAC | |
| m: TGTCAC | ||||
| G1010A | 8 | n: CACGGG | BscGI: CCCGT | |
| m: CACAGG | (− strand) | |||
| 1060delAAG | 8 | n: AGAAGA | MboII: GAAGA | |
| m: AGATGA | ||||
| A1081G | 8 | n: TGCAAC | CviRI: TGCA | |
| m: TGCGAC | ||||
| G1127T | 9 | n: GAGCATGA | BspHI: TCATGA | |
| m: GATCATGA | Mbo I; Dpn I: Sau3AI: GATC | |||
| G1174A | 9 | n: GTCGCC | MaeIII: GTNAC | |
| m: GTCACC | ||||
| 1203insAGC | 9 | n: CTGCAT | Pst I: CTGCAG | |
| m: CTGCAGCA | Bby I: GCAGC | |||
| G1281T | 10 | n: GGAGGC | Mnl I: CCTC | Fok I: GGATG |
| m: GGATGC | (− strand) | |||
| C1454T | 11 | n: GTCTCG | BsmAI: GTCTC | |
| m: GTTTCG | ||||
| C1456T | 11 | n: GTCTCG | BsmAI: GTCTC | |
| m: GTCTTG | ||||
| G1493A | 11 | n: CACCCGCTC | AccBSI: GAGCGG | TaqII: |
| m: CACCCACTC | (− strand); Aci I: CCGC | CACCCA(n)11 | ||
| G1529A | 11 | n: CCGAGG | Sty I: CCWWGG | |
| m: CCAAGG | ||||
| C1594T | 11 | n: GCCGGG | Msp I; HpaII: CCGG | |
| m: GCTGGG | ScrFI: CCNGG |
| Mutation . | Exon . | Recognition Sequence . | Restriction Enzyme for Normal Site . | Restriction Enzyme for Mutated Site . |
|---|---|---|---|---|
| 101-1G-A | n: gGGCCA | Sau96I: GGNCC | Hae I: WGGCCW | |
| m: aGGCCA | ||||
| 628delGT | 6 | n: CCGGGTCG | ScrFI: CCNGG | |
| m: CCGGCG | Nci I; Bcn I: CCSGG | |||
| G787A | 7 | n: CCCGGG | Sma I; Xma I: CCCGGG | EcoRII: CCWGG |
| m: CCCAGG | Msp I; HpaII: CCGG | |||
| G994A | 8 | n: GACGGC | No restriction site found | No restriction site found |
| m: GACAGC | ||||
| G1006T | 8 | n: TGGCAC | MaeIII: GTNAC | |
| m: TGTCAC | ||||
| G1010A | 8 | n: CACGGG | BscGI: CCCGT | |
| m: CACAGG | (− strand) | |||
| 1060delAAG | 8 | n: AGAAGA | MboII: GAAGA | |
| m: AGATGA | ||||
| A1081G | 8 | n: TGCAAC | CviRI: TGCA | |
| m: TGCGAC | ||||
| G1127T | 9 | n: GAGCATGA | BspHI: TCATGA | |
| m: GATCATGA | Mbo I; Dpn I: Sau3AI: GATC | |||
| G1174A | 9 | n: GTCGCC | MaeIII: GTNAC | |
| m: GTCACC | ||||
| 1203insAGC | 9 | n: CTGCAT | Pst I: CTGCAG | |
| m: CTGCAGCA | Bby I: GCAGC | |||
| G1281T | 10 | n: GGAGGC | Mnl I: CCTC | Fok I: GGATG |
| m: GGATGC | (− strand) | |||
| C1454T | 11 | n: GTCTCG | BsmAI: GTCTC | |
| m: GTTTCG | ||||
| C1456T | 11 | n: GTCTCG | BsmAI: GTCTC | |
| m: GTCTTG | ||||
| G1493A | 11 | n: CACCCGCTC | AccBSI: GAGCGG | TaqII: |
| m: CACCCACTC | (− strand); Aci I: CCGC | CACCCA(n)11 | ||
| G1529A | 11 | n: CCGAGG | Sty I: CCWWGG | |
| m: CCAAGG | ||||
| C1594T | 11 | n: GCCGGG | Msp I; HpaII: CCGG | |
| m: GCTGGG | ScrFI: CCNGG |
Boldface letters designate mutated nts.
Abbreviations: n, normal; m, mutated.