Marked Decrease in the Number of Apparent Skeletal Units With Increase in Their Size in the Homozygous Protein 4.1 (−) Madrid
| Apparent Cytoskeletal Units . | Normal . | Protein 4.1 (−) Madrid . |
|---|---|---|
| . | (n = 20) . | (n = 1) . |
| No. (/μm2) | 548 ± 39 | 131 ± 21 |
| Size (%) in diameter of the longer axis | ||
| Small (20-44 nm) | 70 ± 10 | 13 ± 4 |
| Medium (45-68 nm) | 25 ± 6 | 23 ± 6 |
| Large (69-92 nm) | 5 ± 1 | 27 ± 10 |
| Extra-large (93-240 nm) | 0 | 37 ± 15 |
| Apparent Cytoskeletal Units . | Normal . | Protein 4.1 (−) Madrid . |
|---|---|---|
| . | (n = 20) . | (n = 1) . |
| No. (/μm2) | 548 ± 39 | 131 ± 21 |
| Size (%) in diameter of the longer axis | ||
| Small (20-44 nm) | 70 ± 10 | 13 ± 4 |
| Medium (45-68 nm) | 25 ± 6 | 23 ± 6 |
| Large (69-92 nm) | 5 ± 1 | 27 ± 10 |
| Extra-large (93-240 nm) | 0 | 37 ± 15 |
RBC ghost membranes were subjected to EM studies using the QFDE method. The number of apparent skeletal units in the homozygous protein 4.1 (−) Madrid (n = 1, obs 52) as compared with that in the normal subjects (n = 20, obs 272) was counted. The results are shown as the number of apparent skeletal units per square micrometer. The size distribution of these skeletal units was also evaluated, and the results are shown in percentages. Mean values and 1 SD are shown.