Membrane Protein Profiles in Protein 4.1 (−) Madrid
| Proteins . | Normal Subjects . | Protein 4.1 (−) Madrid . |
|---|---|---|
| . | Mean ± 1 SD . | Average . |
| . | (n = 13) . | (n = 2) . |
| On the Fairbanks gels | ||
| Spectrins (α + β)/band 3 | 85.8 ± 3.5 | 67.4 (−21.4%*) |
| Ankyrin/band 3 | 13.3 ± 1.0 | 14.0 |
| On the Laemmli gels | ||
| Band 4.2/band 3 | 14.4 ± 1.1 | 12.9 |
| Actin/band 3 | 14.7 ± 1.2 | 12.0 (−18.4%*) |
| Proteins . | Normal Subjects . | Protein 4.1 (−) Madrid . |
|---|---|---|
| . | Mean ± 1 SD . | Average . |
| . | (n = 13) . | (n = 2) . |
| On the Fairbanks gels | ||
| Spectrins (α + β)/band 3 | 85.8 ± 3.5 | 67.4 (−21.4%*) |
| Ankyrin/band 3 | 13.3 ± 1.0 | 14.0 |
| On the Laemmli gels | ||
| Band 4.2/band 3 | 14.4 ± 1.1 | 12.9 |
| Actin/band 3 | 14.7 ± 1.2 | 12.0 (−18.4%*) |
SDS-PAGE was performed in the protein 4.1 (−) Madrid case on two independent occasions in triplicate. Results were compared with those in normal subjects (n = 13). Each membrane protein band stained with Coomassie blue was determined densitometrically at 570 nm after SDS-PAGE was performed, as previously described.39 The relative ratios are given as percentages, and mean values ± 1 SD are shown.
Statistically significant reduction (P < .05).