Distribution of Neutrophils, Neutrophil Precursors, and Progenitor Cells by Strain Type in Response to G-CSF at 200 μg/kg/d for 5 Days
| Organ . | Cell Type . | Total Cell Numbers . | ||
|---|---|---|---|---|
| . | . | C57BL/6 . | BABL/c . | DBA/2 . |
| Baseline | ||||
| PB | Neutrophils (×10−6) | 1.8 ± 0.6 | 2.0 ± 0.8 | 2.2 ± 0.7 |
| Myeloid CFC (×10−3) | 0.06 ± 0.1 | 0.05 ± 0.1 | 0.2 ± 0.2 | |
| BM | Neutrophils (×10−6) | 132 ± 4 | 100 ± 10* | 124 ± 3 |
| Neutrophil precursors (×10−6) | 187 ± 6 | 175 ± 15 | 150 ± 9 | |
| Myeloid CFC (×10−3) | 645 ± 103 | 658 ± 233 | 768 ± 173 | |
| Spleen | Neutrophils (×10−6) | 4 ± 1 | 11 ± 3 | 5 ± 2 |
| Neutrophil precursors (×10−6) | 8 ± 1 | 17 ± 3* | 9 ± 2 | |
| Myeloid CFC (×10−3) | 4 ± 4 | 3 ± 3 | 5 ± 4 | |
| Total body | Neutrophils (×10−6) | 138 ± 5 | 113 ± 13 | 131 ± 7 |
| Neutrophil precursors (×10−6) | 195 ± 7 | 192 ± 18 | 159 ± 11 | |
| Myeloid CFC (×10−3) | 651 ± 107 | 663 ± 236 | 773 ± 177 | |
| Post G-CSF | ||||
| PB | Neutrophils (×10−6) | 38 ± 1 | 35 ± 12 | 65 ± 18* |
| Myeloid CFC (×10−3) | 2 ± 1* | 9 ± 3* | 24 ± 3* | |
| BM | Neutrophils (×10−6) | 233 ± 24* | 107 ± 10 | 130 ± 3 |
| Neutrophil precursors (×10−6) | 368 ± 34* | 221 ± 30 | 186 ± 10 | |
| Myeloid CFC (×10−3) | 590 ± 263 | 533 ± 167 | 447 ± 66 | |
| Spleen | Neutrophils (×10−6) | 90 ± 34 | 82 ± 28 | 125 ± 36 |
| Neutrophil precursors (×10−6) | 117 ± 25 | 142 ± 30 | 164 ± 46 | |
| Myeloid CFC (×10−3) | 214 ± 172 | 493 ± 152 | 860 ± 312* | |
| Total body | Neutrophils (×10−6) | 361 ± 69 | 224 ± 50 | 320 ± 57 |
| Neutrophil precursors (×10−6) | 486 ± 68 | 367 ± 58 | 350 ± 56 | |
| Myeloid CFC (×10−3) | 806 ± 436 | 1,035 ± 332 | 1,331 ± 378 | |
| Organ . | Cell Type . | Total Cell Numbers . | ||
|---|---|---|---|---|
| . | . | C57BL/6 . | BABL/c . | DBA/2 . |
| Baseline | ||||
| PB | Neutrophils (×10−6) | 1.8 ± 0.6 | 2.0 ± 0.8 | 2.2 ± 0.7 |
| Myeloid CFC (×10−3) | 0.06 ± 0.1 | 0.05 ± 0.1 | 0.2 ± 0.2 | |
| BM | Neutrophils (×10−6) | 132 ± 4 | 100 ± 10* | 124 ± 3 |
| Neutrophil precursors (×10−6) | 187 ± 6 | 175 ± 15 | 150 ± 9 | |
| Myeloid CFC (×10−3) | 645 ± 103 | 658 ± 233 | 768 ± 173 | |
| Spleen | Neutrophils (×10−6) | 4 ± 1 | 11 ± 3 | 5 ± 2 |
| Neutrophil precursors (×10−6) | 8 ± 1 | 17 ± 3* | 9 ± 2 | |
| Myeloid CFC (×10−3) | 4 ± 4 | 3 ± 3 | 5 ± 4 | |
| Total body | Neutrophils (×10−6) | 138 ± 5 | 113 ± 13 | 131 ± 7 |
| Neutrophil precursors (×10−6) | 195 ± 7 | 192 ± 18 | 159 ± 11 | |
| Myeloid CFC (×10−3) | 651 ± 107 | 663 ± 236 | 773 ± 177 | |
| Post G-CSF | ||||
| PB | Neutrophils (×10−6) | 38 ± 1 | 35 ± 12 | 65 ± 18* |
| Myeloid CFC (×10−3) | 2 ± 1* | 9 ± 3* | 24 ± 3* | |
| BM | Neutrophils (×10−6) | 233 ± 24* | 107 ± 10 | 130 ± 3 |
| Neutrophil precursors (×10−6) | 368 ± 34* | 221 ± 30 | 186 ± 10 | |
| Myeloid CFC (×10−3) | 590 ± 263 | 533 ± 167 | 447 ± 66 | |
| Spleen | Neutrophils (×10−6) | 90 ± 34 | 82 ± 28 | 125 ± 36 |
| Neutrophil precursors (×10−6) | 117 ± 25 | 142 ± 30 | 164 ± 46 | |
| Myeloid CFC (×10−3) | 214 ± 172 | 493 ± 152 | 860 ± 312* | |
| Total body | Neutrophils (×10−6) | 361 ± 69 | 224 ± 50 | 320 ± 57 |
| Neutrophil precursors (×10−6) | 486 ± 68 | 367 ± 58 | 350 ± 56 | |
| Myeloid CFC (×10−3) | 806 ± 436 | 1,035 ± 332 | 1,331 ± 378 | |
Values represent means ± SD of results from three mice per group at baseline and four mice per group after G-CSF. The numbers of neutrophils, neutrophil precursors (metamyelocytes, myelocytes, promyelocytes, and myeloblasts), and myeloid progenitor cells per tissue were calculated from the total cellularity of each tissue and the relative frequencies of each cell type within these populations, as described in the Materials and Methods. The peripheral blood volume was assumed to be 2 mL per mouse and a whole femur to be equivalent to 6% of the total bone marrow.28 Progenitor cell cultures were stimulated by SCM.
Abbreviations: PB, peripheral blood; BM, bone marrow.
Results were significantly different from the results of other strains. Results were initially compared by ANOVA; if P < .01, pairwise analyses (Student-Newman-Keuls test) were performed and considered significant if P < .05.