Evaluation of the Number of Megakaryocytic Colonies in a Fibrinclot Serum-Free Fibrinclot Assay
| . | No. of CFU-meg/5 × 103 CD34+ Cells . |
|---|---|
| Medium alone | 1.5 ± 1 |
| TPO (100 ng/mL) | 36 ± 6.5 |
| TPO (100 ng/mL) + RGDS | 20 ± 4* |
| TPO (100 ng/mL) + RGES | 41 ± 8 |
| TPO (100 ng/mL) + 402-411 γ peptide | 18.6 ± 5* |
| TPO (100 ng/mL) + anti-αIIb | 21 ± 6.4* |
| TPO (100 ng/mL) + anti-αvβ3 | 33 ± 8.8 |
| . | No. of CFU-meg/5 × 103 CD34+ Cells . |
|---|---|
| Medium alone | 1.5 ± 1 |
| TPO (100 ng/mL) | 36 ± 6.5 |
| TPO (100 ng/mL) + RGDS | 20 ± 4* |
| TPO (100 ng/mL) + RGES | 41 ± 8 |
| TPO (100 ng/mL) + 402-411 γ peptide | 18.6 ± 5* |
| TPO (100 ng/mL) + anti-αIIb | 21 ± 6.4* |
| TPO (100 ng/mL) + anti-αvβ3 | 33 ± 8.8 |
Data represent the means ± SD of five separate experiments performed in triplicate.
Statistically significant (P < .05) differences between cultures stimulated with TPO alone or TPO plus various peptides.