Enhanced Lymphoid Clonogenic Potential of IL-7Rα+ Progenitors
| Fraction . | Colonies/10,000 Cells (Cloning Efficiency) . | % Colonies in Sorted Fraction* . |
|---|---|---|
| IL-7Rα+/CD19− | 448 ± 213† | 61.2 ± 8.4 |
| IL-7Rα−/CD19− | 9 ± 3 | 37.7 ± 7.8 |
| IL-7Rα+/CD19+ | 8 ± 8 | 0.9 ± 0.9 |
| IL-7Rα−/CD19+ | 0.5 ± 0.5 | 0.2 ± 0.2 |
| Fraction . | Colonies/10,000 Cells (Cloning Efficiency) . | % Colonies in Sorted Fraction* . |
|---|---|---|
| IL-7Rα+/CD19− | 448 ± 213† | 61.2 ± 8.4 |
| IL-7Rα−/CD19− | 9 ± 3 | 37.7 ± 7.8 |
| IL-7Rα+/CD19+ | 8 ± 8 | 0.9 ± 0.9 |
| IL-7Rα−/CD19+ | 0.5 ± 0.5 | 0.2 ± 0.2 |
The percentage of total lymphoid progenitor colonies in each sorted fraction was calculated from the number of colonies/10,000 sorted cells multiplied by the relative size of each sorted fraction within the total CD34+ population.
All results are the mean ± SEM as an estimate of variation of means of 3 replicate wells assayed 3 times. Cloning efficiency of IL-7Rα+/CD19− fraction was significantly higher than all other fractions (P < .05, paired t-test). Percent total colonies in the sorted IL-7Rα+/CD19− fraction is higher than in the IL-7Rα−/CD19− fraction at P = .07 (t-test).