Neutrophil adhesion to fibrinogen/fibrin-coated surfaces was measured under flow conditions or under static conditions. Surfaces with plasma fibrin, pure fibrinogen, and pure fibrin were obtained as described in the Materials and Methods. For adhesion under flow conditions, PMNs (2 × 10⁶ cells/mL HEPES buffer) were perfused at shear stresses of 80 mPa (5 minutes at 37°C). Adhesion to fibrin under static conditions was measured after a PMN incubation for 45 minutes at 37°C (2 × 10⁶ cells/mL HEPES buffer) with or without PMA (100 ng/mL). Data represent the number of adhering PMNs per square millimeter (mean ± SE, n = 3 to 6).

Abbreviation: ND, not determined.

Statistically significant effect of PMA activation was determined by paired Student's t-test (P < .05).