Progressive Expansion of CFU-Bl in LTCs
| Weeks of . | Blast Cell Colonies . | ||
|---|---|---|---|
| LTC . | Fold Increase . | % Recloning . | No. of Secondary Colonies/ . |
| . | . | Efficiency3-150 . | Blast Colony . |
| 0 | 1 | 63 ± 5.4 | 5.6 ± 1.2 |
| +3 | 9.24 ± 0.6 | 55 ± 2.6 | 5.2 ± 0.4 |
| +5 | 43 ± 2.7 | ND | ND |
| +8 | 421 ± 11 | ND | ND |
| +10 | 4,200 ± 97 | 61 ± 3.1 | 4.3 ± 1.2 |
| +12 | 5,407 ± 145 | ND | ND |
| +15 | 28,672 ± 1,200 | 58 ± 2.3 | 6 ± 0.8 |
| +18 | 65,556 ± 2,406 | ND | ND |
| +20 | 305,420 ± 12,400 | 53 ± 4.6 | 6.7 ± 1.2 |
| Weeks of . | Blast Cell Colonies . | ||
|---|---|---|---|
| LTC . | Fold Increase . | % Recloning . | No. of Secondary Colonies/ . |
| . | . | Efficiency3-150 . | Blast Colony . |
| 0 | 1 | 63 ± 5.4 | 5.6 ± 1.2 |
| +3 | 9.24 ± 0.6 | 55 ± 2.6 | 5.2 ± 0.4 |
| +5 | 43 ± 2.7 | ND | ND |
| +8 | 421 ± 11 | ND | ND |
| +10 | 4,200 ± 97 | 61 ± 3.1 | 4.3 ± 1.2 |
| +12 | 5,407 ± 145 | ND | ND |
| +15 | 28,672 ± 1,200 | 58 ± 2.3 | 6 ± 0.8 |
| +18 | 65,556 ± 2,406 | ND | ND |
| +20 | 305,420 ± 12,400 | 53 ± 4.6 | 6.7 ± 1.2 |
Ability of FL + TPO to support CFU-Bl in LTC. CFU-Bl generated by 1,000 CD34+ CB cells at the beginning of the LTC (time 0) and secondary colonies after replating (mean ± SEM values from 3 separate experiments). Secondary clones comprised all types of colonies (GM, G, erythroid, burst, and GEMM clones represented the total numbers of colonies, respectively).
The recloning efficiency is the percentage of colonies that generates secondary colonies upon replating. Results represent the mean ± SEM of three separate experiments.