Table 7. Nearly All CD34 MM B Cells... | American Society of Hematology

Table 7.

Nearly All CD34⁺ MM B Cells Express Clonotypic IgH VDJ mRNA

PBMC Donor	Subset	% Clonotypic Cells
MM Pt 1	CD34⁺19⁺	91 (971/1,065)
MM Pt 2	CD34⁺19⁺	94 (705/747)
MM Pt 8	CD34⁺19⁺	88 (363/413)
MM Pt 9	CD34⁺19⁺	74 (180/243)
		Mean = 87 ± 5
Unrelated MM Pts	CD19⁺	<0.2
Normals	CD19⁺	<0.3

PBMC Donor	Subset	% Clonotypic Cells
MM Pt 1	CD34⁺19⁺	91 (971/1,065)
MM Pt 2	CD34⁺19⁺	94 (705/747)
MM Pt 8	CD34⁺19⁺	88 (363/413)
MM Pt 9	CD34⁺19⁺	74 (180/243)
		Mean = 87 ± 5
Unrelated MM Pts	CD19⁺	<0.2
Normals	CD19⁺	<0.3

Each set of patient-specific primers was tested on sorted CD19⁺ B cells from at least one unrelated MM donor and one normal donor. In no case were positive cells detected. For 3/4 MM Pts, the number of CD34⁺19⁺ B cells with clonotypic mRNA was comparable to the number having histone mRNA, CD19 mRNA, or total IgH mRNA (consensus primers), suggesting that all B cells with intact mRNA had clonotypic mRNA; for the fourth patient, insufficient slides were available to evaluate other primers.

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