CD19+ B Cells From PBMC of MM Patients Express CD34 mRNA as Well as mRNA for CD19 and IgH CDR3: In Situ RT-PCR
| Source of CD19+ PBMC . | % Positive . | CD34+ Cells/L Blood Absolute No. . | |||
|---|---|---|---|---|---|
| . | CD34 (no. pos/tot) . | CD19 . | IgHVDJ . | Histone . | . |
| MM patients (treatment status) | |||||
| 1 (Unt) | 85 (157/184) | 95 | 0.69 × 109 | ||
| 2-a (Unt) | 27 (111/401) | 0.06 × 109 | |||
| 2-b (Tr) | 61 (164/269) | 94 | 97 | 90 | 0.08 × 109 |
| 3 (Tr) | 78 (736/942) | 88 | 93 | 90 | 0.18 × 109 |
| 4 (Tr) | 73 (329/452) | 78 | 96 | 99 | 0.24 × 109 |
| 5 (Tr) | 82 (324/392) | 96 | 95 | 0.29 × 109 | |
| 6-a (Off Tr) | 64 (251/395) | 97 | 97 | 94 | 0.14 × 109 |
| 6-b (Off Tr) | 53 (81/153) | 0.10 × 109 | |||
| Mean values | 65 ± 7 | 89 ± 4 | 96 ± .6 | 94 ± 1 | 0.22 ± .07 × 109 |
| Normal donors (3) | 0.8 ± .7 (0.3-1.8)5-150 | 92 | 97 | 90 ± 3 | |
| Source of CD19+ PBMC . | % Positive . | CD34+ Cells/L Blood Absolute No. . | |||
|---|---|---|---|---|---|
| . | CD34 (no. pos/tot) . | CD19 . | IgHVDJ . | Histone . | . |
| MM patients (treatment status) | |||||
| 1 (Unt) | 85 (157/184) | 95 | 0.69 × 109 | ||
| 2-a (Unt) | 27 (111/401) | 0.06 × 109 | |||
| 2-b (Tr) | 61 (164/269) | 94 | 97 | 90 | 0.08 × 109 |
| 3 (Tr) | 78 (736/942) | 88 | 93 | 90 | 0.18 × 109 |
| 4 (Tr) | 73 (329/452) | 78 | 96 | 99 | 0.24 × 109 |
| 5 (Tr) | 82 (324/392) | 96 | 95 | 0.29 × 109 | |
| 6-a (Off Tr) | 64 (251/395) | 97 | 97 | 94 | 0.14 × 109 |
| 6-b (Off Tr) | 53 (81/153) | 0.10 × 109 | |||
| Mean values | 65 ± 7 | 89 ± 4 | 96 ± .6 | 94 ± 1 | 0.22 ± .07 × 109 |
| Normal donors (3) | 0.8 ± .7 (0.3-1.8)5-150 | 92 | 97 | 90 ± 3 | |
PBMC from MM patients or normal donors were stained with CD19 MoAb and sorted for positive cells. On reanalysis >96% of sorted cells were positive for CD19 staining. For some samples very few slides were available for this analysis. T cells from MM patients had 0% to 8% of cells that were weakly positive for CD34 mRNA, and none that had an accumulation of colored product comparable to that seen for B cells (not shown), and lacked IgH or CD19 mRNA.9 10 For each patient, >150 cells were microscopically counted to determine the number positive for CD19, IgH, and histone in situ RT-PCR staining. Replicate staining, when possible, gave essentially the same values on each slide. Mean values are ± SE.
Range of values.