Oligonucleotide Primers Used for RT-PCR
| Gene . | GenEMBL Bank Accession No. . | Primer Pairs . | Length of PCR Product (bp) . |
|---|---|---|---|
| EPO | M12930 | L: | 339 |
| 5′-AAGGAGGCAGAAAATGTCACGATG-3′ | |||
| R: 5′-ACCTGCTCCACTCCGAACACTCAC-3′ | |||
| EPO-R | J04843 | L: | 497 |
| 5′-CAATGAAGTAGTGCTCCTGGAC-3′ | |||
| R: 5′-GAGATGAGGACCAGAATGAGAG-3′ | |||
| b-FGF-R | M28998 | L: | 236 |
| 5′-ATGTGCAGAGCATCAACTGG-3′ | |||
| R: 5′-TCGTCGTCGTCATCATCTTC-3′ | |||
| GATA-1 | X03221 | L: | 367 |
| 5′-TGTGGTCTACAGCCTCTCAGCAC-3′ | |||
| R: 5′-GTGTAGTTCAAGTGAGGCAGAG-3′ | |||
| GATA-2 | M77810 | L: | 528 |
| 5′-TCTCAATCACCTCGACTCG-3′ | |||
| R: 5′-GACGGAGAGCATGAAGATG-3′ | |||
| Flk-2/Flt-3 | M64689 | L: | 299 |
| 5′-CTTCTGGTCAAATGCTGTGC-3′ | |||
| R: 5′-GGGAGTTCCGAGAGAGAAC-3′ | |||
| GM-CSF | X03221 | L: | 367 |
| 5′-TGTGGTCTACAGCCTCTCAGCAC-3′ | |||
| R: 5′-GTGTAGTTCAAGTGAGGCAGAG-3′ | |||
| Hox B5 | M26283 | L: | 485 |
| 5′-ATTATGGCAGTGGCAGCTCT-3′ | |||
| R: 5′-TGTGAAGCTTCCTCATCCAG-3′ | |||
| Hox B6 | J30782 | L: | 469 |
| 5′-CTGGAGCTGGAGAAGGAATT-3′ | |||
| R: 5′-TCCGATTCTCAGTTCTCACC-3′ | |||
| Hox B7 | Y00436 | L: | 164 |
| 5′-AAGCGAG&CCGCCAGACCTACACG-3′ | |||
| R: 5′-CCACTTCATGCGCCGGTTCTGAA-3′ | |||
| Hox B8 | X13721 | L: | 489 |
| 5′-AATCCAGGAGTTCTACCACG-3′ | |||
| R: 5′-GCAACTTCTACGGCTACCAC-3′ | |||
| HPRT | J00423 | L: | 229 |
| 5′-CACAGGACTAGAACACCTGC-3′ | |||
| R: 5′-GCTGGTGAAAAGGACCTCT-3′ | |||
| LIF-R | D17444 | L: | 460 |
| 5′-TGACTTCTTCACCTCCTCCT-3′ | |||
| R: 5′-TACAGATGGATGAGAGGACG-3′ | |||
| SCF | M59915 | L: | 320 |
| 5′-GCTTGACTACTCTTCTGGAC-3′ | |||
| R: 5′-GTGACACTGACTCGTGAATC-3′ | |||
| SCF-R | Y00864 | L: | 490 |
| 5′-TGTGTCTACATCCGTGAACTCC-3′ | |||
| R: 5′-GGTATAAGTGCCTCCTTCTGTG-3′ | |||
| CD34 | M81104 | L: | 618 |
| 5′-GTTACCTCTGGGATCCCTTCAGGCTC-3′ | |||
| R: 5′-GMTMCGTAACCAGTGGAGACCTC-3′ |
| Gene . | GenEMBL Bank Accession No. . | Primer Pairs . | Length of PCR Product (bp) . |
|---|---|---|---|
| EPO | M12930 | L: | 339 |
| 5′-AAGGAGGCAGAAAATGTCACGATG-3′ | |||
| R: 5′-ACCTGCTCCACTCCGAACACTCAC-3′ | |||
| EPO-R | J04843 | L: | 497 |
| 5′-CAATGAAGTAGTGCTCCTGGAC-3′ | |||
| R: 5′-GAGATGAGGACCAGAATGAGAG-3′ | |||
| b-FGF-R | M28998 | L: | 236 |
| 5′-ATGTGCAGAGCATCAACTGG-3′ | |||
| R: 5′-TCGTCGTCGTCATCATCTTC-3′ | |||
| GATA-1 | X03221 | L: | 367 |
| 5′-TGTGGTCTACAGCCTCTCAGCAC-3′ | |||
| R: 5′-GTGTAGTTCAAGTGAGGCAGAG-3′ | |||
| GATA-2 | M77810 | L: | 528 |
| 5′-TCTCAATCACCTCGACTCG-3′ | |||
| R: 5′-GACGGAGAGCATGAAGATG-3′ | |||
| Flk-2/Flt-3 | M64689 | L: | 299 |
| 5′-CTTCTGGTCAAATGCTGTGC-3′ | |||
| R: 5′-GGGAGTTCCGAGAGAGAAC-3′ | |||
| GM-CSF | X03221 | L: | 367 |
| 5′-TGTGGTCTACAGCCTCTCAGCAC-3′ | |||
| R: 5′-GTGTAGTTCAAGTGAGGCAGAG-3′ | |||
| Hox B5 | M26283 | L: | 485 |
| 5′-ATTATGGCAGTGGCAGCTCT-3′ | |||
| R: 5′-TGTGAAGCTTCCTCATCCAG-3′ | |||
| Hox B6 | J30782 | L: | 469 |
| 5′-CTGGAGCTGGAGAAGGAATT-3′ | |||
| R: 5′-TCCGATTCTCAGTTCTCACC-3′ | |||
| Hox B7 | Y00436 | L: | 164 |
| 5′-AAGCGAG&CCGCCAGACCTACACG-3′ | |||
| R: 5′-CCACTTCATGCGCCGGTTCTGAA-3′ | |||
| Hox B8 | X13721 | L: | 489 |
| 5′-AATCCAGGAGTTCTACCACG-3′ | |||
| R: 5′-GCAACTTCTACGGCTACCAC-3′ | |||
| HPRT | J00423 | L: | 229 |
| 5′-CACAGGACTAGAACACCTGC-3′ | |||
| R: 5′-GCTGGTGAAAAGGACCTCT-3′ | |||
| LIF-R | D17444 | L: | 460 |
| 5′-TGACTTCTTCACCTCCTCCT-3′ | |||
| R: 5′-TACAGATGGATGAGAGGACG-3′ | |||
| SCF | M59915 | L: | 320 |
| 5′-GCTTGACTACTCTTCTGGAC-3′ | |||
| R: 5′-GTGACACTGACTCGTGAATC-3′ | |||
| SCF-R | Y00864 | L: | 490 |
| 5′-TGTGTCTACATCCGTGAACTCC-3′ | |||
| R: 5′-GGTATAAGTGCCTCCTTCTGTG-3′ | |||
| CD34 | M81104 | L: | 618 |
| 5′-GTTACCTCTGGGATCCCTTCAGGCTC-3′ | |||
| R: 5′-GMTMCGTAACCAGTGGAGACCTC-3′ |