Progenitor Cell Content in Freshly Isolated Yolk Sacs and in Bone Marrow From Secondary Recipient and Normal Control Animals
| Cell Source . | Colonies/100,000 Cells Plated . | ||||
|---|---|---|---|---|---|
| . | E . | G . | M . | GM . | GEMM . |
| Day-10 yolk sac | 236 ± 366-150 | 0 | 163 ± 196-150 | 22 ± 8 | 121 ± 126-150 |
| Secondary host | 33 ± 17 | 121 ± 21 | 95 ± 26 | 16 ± 9 | 67 ± 25 |
| Normal control | 39 ± 12 | 116 ± 18 | 101 ± 12 | 28 ± 13 | 59 ± 21 |
| Cell Source . | Colonies/100,000 Cells Plated . | ||||
|---|---|---|---|---|---|
| . | E . | G . | M . | GM . | GEMM . |
| Day-10 yolk sac | 236 ± 366-150 | 0 | 163 ± 196-150 | 22 ± 8 | 121 ± 126-150 |
| Secondary host | 33 ± 17 | 121 ± 21 | 95 ± 26 | 16 ± 9 | 67 ± 25 |
| Normal control | 39 ± 12 | 116 ± 18 | 101 ± 12 | 28 ± 13 | 59 ± 21 |
Burst-forming unit-erythroid (E) and colony-forming units-granulocyte (G), -macrophage (M), –granulocyte-macrophage (GM), and mixed lineage (GEMM) were scored after 7 days of culture and colony identity was confirmed by plucking colonies and microscopically identifying the Giemsa-stained mature blood cells present. Data shown are the mean ± SD of triplicate cultures of two experiments.
P < .01 day 10 yolk sac versus secondary host or normal control.