Release of Antiinflammatory Cytokines Into Whole Blood
| . | Control (n = 6) . | Patient (n = 6) . | ||
|---|---|---|---|---|
| . | −LPS . | +LPS . | −LPS . | +LPS . |
| IL-4 (pg/mL) | ND | 7.8 ± 7.8 | 21.3 ± 11.2 | 22.7 ± 16.55-150 |
| IL-10 (pg/mL) | 68.0 ± 36.0 | 1,818.7 ± 394.9 | 123.8 ± 54.8 | 147.8 ± 41.75-150 |
| TGF-β1 (pg/mL) | 25,757 ± 1,256 | 28,771 ± 3,594 | 9,648 ± 2,2195-150 | 13,314 ± 2,6125-150 |
| . | Control (n = 6) . | Patient (n = 6) . | ||
|---|---|---|---|---|
| . | −LPS . | +LPS . | −LPS . | +LPS . |
| IL-4 (pg/mL) | ND | 7.8 ± 7.8 | 21.3 ± 11.2 | 22.7 ± 16.55-150 |
| IL-10 (pg/mL) | 68.0 ± 36.0 | 1,818.7 ± 394.9 | 123.8 ± 54.8 | 147.8 ± 41.75-150 |
| TGF-β1 (pg/mL) | 25,757 ± 1,256 | 28,771 ± 3,594 | 9,648 ± 2,2195-150 | 13,314 ± 2,6125-150 |
Heparinized whole blood obtained from healthy individuals (controls, n = 6) and critically ill patients (n = 6) was incubated with or without LPS (1 ng/mL) for 24 hours. Plasma levels of IL-4, 10, and TGF-β1 were measured using specific ELISA. Data are presented as mean ± SEM.
Abbreviation: ND, not detectable.
P < .05 patient versus control.