Table 4. Effect of Trauma and Sepsis on... | American Society of Hematology

Table 4.

Effect of Trauma and Sepsis on Release of IL-12 p40, p70, and IFN-γ Into Whole Blood

	Control (n = 12)			Trauma (n = 17)			Sepsis (n = 8)
	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70
p40	964.9 ± 185.8	1,673.7 ± 254.5^4-150	—	48.1 ± 7.1^4-151	256.2 ± 58.4^*†	—	61.3 ± 14.5^4-151	161.4 ± 79.5^4-151	—
p70	9.0 ± 3.5	299.8 ± 105.1^4-150	—	ND^4-151	ND^4-151	—	ND^4-151	ND^4-151	—
IFN-γ	1,301.8 ± 186.6	—	3,223.8 ± 527.8^4-150	320.0 ± 74.7^4-151	—	321.3 ± 98.8^4-151	187.7 ± 45.5^4-151	—	205.2 ± 72.7^4-151

	Control (n = 12)			Trauma (n = 17)			Sepsis (n = 8)
	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70	LPS	LPS + rhIFN-γ	LPS + rhIL-12 p70
p40	964.9 ± 185.8	1,673.7 ± 254.5^4-150	—	48.1 ± 7.1^4-151	256.2 ± 58.4^*†	—	61.3 ± 14.5^4-151	161.4 ± 79.5^4-151	—
p70	9.0 ± 3.5	299.8 ± 105.1^4-150	—	ND^4-151	ND^4-151	—	ND^4-151	ND^4-151	—
IFN-γ	1,301.8 ± 186.6	—	3,223.8 ± 527.8^4-150	320.0 ± 74.7^4-151	—	321.3 ± 98.8^4-151	187.7 ± 45.5^4-151	—	205.2 ± 72.7^4-151

Whole blood obtained from healthy individuals (controls, n = 12) and critically ill patients (n = 25) was incubated with LPS (1 ng/mL) in the presence or absence of either rhIL-12 p70 (5 ng/mL) or rhIFN-γ (10 ng/mL) for 24 hours. The group of critically ill patients was divided according to the origin of the insult (trauma v sepsis). Plasma levels of IL-12 p40 (pg/mL), p70 (pg/mL), and IFN-γ (pg/mL) were measured using specific ELISA. Data are presented as mean ± SEM.

Abbreviation: ND, not detectable.

F4-150

P < .05 LPS versus LPS + rhIFN-γ/LPS + rhIL-12 p70.

F4-151

P < .05 patient versus control.

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