Secretion of IFN-γ Into Whole Blood
| . | . | . | rhIL-12 p70 . | LPS . | LPS + rhIL-12 p70 . |
|---|---|---|---|---|---|
| 8 h | Control | 613.9 ± 155.0 | 1,241.3 ± 217.93-150 | 1,255.0 ± 179.83-150 | 2,690.8 ± 595.63-150 |
| Patient | 244.0 ± 51.83-151 | 442.7 ± 117.43-151 | 314.6 ± 67.93-151 | 333.7 ± 86.03-151 | |
| 24 h | Control | 712.4 ± 188.7 | 1,458.1 ± 217.63-150 | 1,301.8 ± 186.63-150 | 3,223.8 ± 527.83-150 |
| Patient | 327.3 ± 86.7 | 328.4 ± 87.73-151 | 278.2 ± 54.23-151 | 282.6 ± 70.03-151 |
| . | . | . | rhIL-12 p70 . | LPS . | LPS + rhIL-12 p70 . |
|---|---|---|---|---|---|
| 8 h | Control | 613.9 ± 155.0 | 1,241.3 ± 217.93-150 | 1,255.0 ± 179.83-150 | 2,690.8 ± 595.63-150 |
| Patient | 244.0 ± 51.83-151 | 442.7 ± 117.43-151 | 314.6 ± 67.93-151 | 333.7 ± 86.03-151 | |
| 24 h | Control | 712.4 ± 188.7 | 1,458.1 ± 217.63-150 | 1,301.8 ± 186.63-150 | 3,223.8 ± 527.83-150 |
| Patient | 327.3 ± 86.7 | 328.4 ± 87.73-151 | 278.2 ± 54.23-151 | 282.6 ± 70.03-151 |
Whole blood obtained from healthy individuals (controls, n = 12) and critically ill patients (n = 25) was incubated with or without LPS (1 ng/mL) in the presence or absence of rhIL-12 p70 (5 ng/mL) for 8 hours and 24 hours, respectively. Plasma levels of IFN-γ (pg/mL) were measured using specific ELISA. Data are presented as mean ± SEM.
P < .05 without agent versus rhIL-12 p70, LPS, or LPS + rhIL-12 p70.
P < .05 patient versus control.